Myeloid and erythroid progenitor content of stromal cultures from CD34+CD38−CD7+ and CD34+CD38−CD7− cells
| Experiment . | CFU-GM* . | CFU-GEMM/BFU-E* . | Total CFU-C* . | |||
|---|---|---|---|---|---|---|
| CD7+ . | CD7− . | CD7+ . | CD7− . | CD7+ . | CD7− . | |
| 1 | 1.0 | 43.0 | 0 | 28.2 | 1.0 | 71.2 |
| 2 | 0.17 | 39.1 | 0 | 0.2 | 0.2 | 39.3 |
| 3 | 0 | 5.5 | 0 | 0.5 | 0 | 6.0 |
| 4 | 0 | 40.3 | 0 | 8.7 | 0 | 49.0 |
| 5 | 0 | 25.1 | 0 | 3.3 | 0 | 28.4 |
| 6 | 0 | 11.9 | 0 | 7.9 | 0 | 19.8 |
| 7 | 0 | 49.0 | 0.5 | 20.6 | 0.5 | 69.6 |
| Mean (± SEM) | 0.17 (0.14) | 30.6 (6.3) | 0.07 (0.07) | 9.9 (4.0) | 0.2 (0.1) | 40.5 (9.3) |
| Pvalue | .003† | .048† | .005† | |||
| Experiment . | CFU-GM* . | CFU-GEMM/BFU-E* . | Total CFU-C* . | |||
|---|---|---|---|---|---|---|
| CD7+ . | CD7− . | CD7+ . | CD7− . | CD7+ . | CD7− . | |
| 1 | 1.0 | 43.0 | 0 | 28.2 | 1.0 | 71.2 |
| 2 | 0.17 | 39.1 | 0 | 0.2 | 0.2 | 39.3 |
| 3 | 0 | 5.5 | 0 | 0.5 | 0 | 6.0 |
| 4 | 0 | 40.3 | 0 | 8.7 | 0 | 49.0 |
| 5 | 0 | 25.1 | 0 | 3.3 | 0 | 28.4 |
| 6 | 0 | 11.9 | 0 | 7.9 | 0 | 19.8 |
| 7 | 0 | 49.0 | 0.5 | 20.6 | 0.5 | 69.6 |
| Mean (± SEM) | 0.17 (0.14) | 30.6 (6.3) | 0.07 (0.07) | 9.9 (4.0) | 0.2 (0.1) | 40.5 (9.3) |
| Pvalue | .003† | .048† | .005† | |||
CFU-GM indicates colony-forming unit–granulocyte-macrophage; CFU-GEMM, colony-forming unit–granulocyte, erythrocyte, megakaryocyte, macrophage; BFU-E, burst-forming unit, erythroid; CFU-C, colony-forming unit–cell.
Shown is the number of each type of CFU-C per 1000 cells plated in methylcellulose.
P value shows significant difference between CD7+ and CD7− populations over 7 experiments.