Rhesus monkey ES cells underwent differentiation in the presence of S17 stromal cells, BMP-4, and hematopoietic growth factors (SCF, IL-3, IL-6, G-CSF, VEGF, Flt3 ligand, and Epo). Reverse transcriptase-polymerase chain reactions used equal amounts of complementary DNA templates adjusted according to the levels of expression of hypoxanthine phosphoribosyltransferase from each sample. For all genes studied, tests with S17 stromal cells yielded negative results with use of the same sets of primers.

A minus sign indicates not expressed; a plus-minus sign, weakly expressed; a plus sign, well expressed; and 2 plus signs, strongly expressed.