Table 1. N-regions and flanking germline... | American Society of Hematology

Table 1.

N-regions and flanking germline sequences in B-cell precursor acute lymphoblastic leukemia patients showing T-cell receptor Vδ2-Dδ3 rearrangements. The sequence used to generate patient-specific clonogenic probes is shown in bold and underlined

Patient	Vδ2	N	Dδ3
Germline	GTGCCTGTGACACC		ACTGGGGGATAC
1	GTGCCTGTGACACC	GGGGAGAGGGTCCCACAC	ACTGGGGGATAC
2	GTGCCTGTGAC	CTCGGGTCCTT	ACTGGGGGATAC
3	GTGCCTGTGACACC	CGGGGGTTCT	TGGGGGATAC
4	GTGCCTGTGAC	CCCCCCCCGAGG	GGGGGATAC
5	GTGCCTGTGAC	CCCCGGAGAACCCCAGGCCGGACAGACAGACTAGT	ACTGGGGGATACGC
6	GTGCCTGTGACACC	TCTGGTACCCTTCGCCCGTTCGCTCCCTAGGCG	TGGGGGATACGC
7	GTGCCTGTGAC	CC	ACTGGGGGATACGC
8	GTGCCTGTGACAC	GGGG	TGGGGGATACGC
9	GTGCCTGT	CTG	GGGGGATACGC
10	GTGCCTGTGA	GGGG	TGGGGGATACGC

Patient	Vδ2	N	Dδ3
Germline	GTGCCTGTGACACC		ACTGGGGGATAC
1	GTGCCTGTGACACC	GGGGAGAGGGTCCCACAC	ACTGGGGGATAC
2	GTGCCTGTGAC	CTCGGGTCCTT	ACTGGGGGATAC
3	GTGCCTGTGACACC	CGGGGGTTCT	TGGGGGATAC
4	GTGCCTGTGAC	CCCCCCCCGAGG	GGGGGATAC
5	GTGCCTGTGAC	CCCCGGAGAACCCCAGGCCGGACAGACAGACTAGT	ACTGGGGGATACGC
6	GTGCCTGTGACACC	TCTGGTACCCTTCGCCCGTTCGCTCCCTAGGCG	TGGGGGATACGC
7	GTGCCTGTGAC	CC	ACTGGGGGATACGC
8	GTGCCTGTGACAC	GGGG	TGGGGGATACGC
9	GTGCCTGT	CTG	GGGGGATACGC
10	GTGCCTGTGA	GGGG	TGGGGGATACGC

Primers used for detection of TCR Vδ2-Dδ3 rearrangements were as described by Campana and coworkers.11 Primer 8: 5′ GAGTCATGTCAGCCATTGAG 3". Primer 9: 5′ GCACCATCAGAGAGAGATGA 3′. Primer 10: 5′ TTGTAGCACTGTGCGTATCC 3′. Primer 11: 5′ AGGGAAATGGCACTTTTGCC 3′. Primers 9 and 10 are nested within the region amplified by primers 8 and 11.

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