Table 2. Yield of CD34 CD38 and CD34... | American Society of Hematology

Table 2.

Yield of CD34⁺CD38⁺ and CD34⁺CD38⁻ cells by FACS and detection of clonotypic T-cell receptor Vδ2-Dδ3 rearrangements in remission marrow

Patient	Remission marrow^*		Detection limit of leukemic cell DNA diluted into normal DNA	Immunophenotype of diagnostic bone marrow		Presence of clonotypic TCR Vδ2-Dδ3 rearrangement in CD34⁺CD38⁻ cells
Patient	No. CD34⁺CD38⁺ cells	No. CD34⁺ CD38⁻cells		% CD34⁺	% CD38⁺
1	20 160	6245	1:10⁵	87	90	+
2	96 736	1970	1:10⁴	27	58	−
3	271 889	1022	1:10⁴	83	90	−
4	261 749	3330	1:10⁶	80	76	+
5	216 307	732	1:10⁴	81	30	−
6	207 308	480	1:10³	48	99	−
7	249 144	583	1:10³	80	97	−
8	472 271	401	1:10⁴	35	97	+
9	271 344	5070	1:10⁴	97	62	+
10	20 006	485	1:10³	3	99	−

Patient	Remission marrow^*		Detection limit of leukemic cell DNA diluted into normal DNA	Immunophenotype of diagnostic bone marrow		Presence of clonotypic TCR Vδ2-Dδ3 rearrangement in CD34⁺CD38⁻ cells
Patient	No. CD34⁺CD38⁺ cells	No. CD34⁺ CD38⁻cells		% CD34⁺	% CD38⁺
1	20 160	6245	1:10⁵	87	90	+
2	96 736	1970	1:10⁴	27	58	−
3	271 889	1022	1:10⁴	83	90	−
4	261 749	3330	1:10⁶	80	76	+
5	216 307	732	1:10⁴	81	30	−
6	207 308	480	1:10³	48	99	−
7	249 144	583	1:10³	80	97	−
8	472 271	401	1:10⁴	35	97	+
9	271 344	5070	1:10⁴	97	62	+
10	20 006	485	1:10³	3	99	−

Shown are numbers of FACS isolated cells from which DNA was extracted. PCR analysis was performed on 100% of cells isolated.