Sequences of oligonucleotide primers used for polymerase chain reaction
| Molecule . | Primer sequence . | PCR product . |
|---|---|---|
| TF | 5′ CTTGTGTAGAGATATAGCCAGG 3′ | 376 bp |
| 5′ GGGAACAAAAGTGAATGTGACC 3′ | ||
| TFPI-1 | 5′ CAACTCTGATACAAACGTGTTGA 3′ | 374 bp |
| 5′ CTACTACAATTCAGTCATTGGGA 3′ | ||
| GAPDH | 5′ GTTGTCATGGATGACCTTGGCC 3′ | 350 bp |
| 5′ CCACCCATGGCAAATTCCATGG 3′ | ||
| E-selectin | 5′ CTCTGACAGAAGAAGCCAAG 3′ | 235 bp |
| 5′ ACTTGAGTCCACTGAAGGCA 3′ |
| Molecule . | Primer sequence . | PCR product . |
|---|---|---|
| TF | 5′ CTTGTGTAGAGATATAGCCAGG 3′ | 376 bp |
| 5′ GGGAACAAAAGTGAATGTGACC 3′ | ||
| TFPI-1 | 5′ CAACTCTGATACAAACGTGTTGA 3′ | 374 bp |
| 5′ CTACTACAATTCAGTCATTGGGA 3′ | ||
| GAPDH | 5′ GTTGTCATGGATGACCTTGGCC 3′ | 350 bp |
| 5′ CCACCCATGGCAAATTCCATGG 3′ | ||
| E-selectin | 5′ CTCTGACAGAAGAAGCCAAG 3′ | 235 bp |
| 5′ ACTTGAGTCCACTGAAGGCA 3′ |
PCR indicates polymerase chain reaction; TF, tissue factor; TFPI-1, tissue factor pathway inhibitor-1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; bp, base pair.