Effects of nicotine and cotinine on adhesion molecule expression in endothelial cells
| Cell population . | FSC/SSC . | Treatment . | Control . | CD44 . | VCAM . | α4 . | β2 . | β7 . | P-selectin . | L-selectin . |
|---|---|---|---|---|---|---|---|---|---|---|
| HUVECs | 369 ± 23/414 ± 12 | NT | 5.7 ± 2/5.3 ± 2 | 95 ± 4/167 ± 63 | 8 ± 3/6 ± 2 | 12 ± 3.4/7 ± 2.3 | 5.7 ± 5.3/7 ± 1 | 8.4 ± 5/7 ± 1.4 | 5.7 ± 1/7 ± 0.3 | 7 ± 4/7 ± 0.7 |
| NC | 4 ± 2/6.7 ± 2.6 | 82 ± 17/79 ± 47 | 7 ± 3/7.5 ± 2.5 | 9.5 ± 2/8.5 ± 3 | 7 ± 4/7 ± 2.6 | 8.6 ± 7/7.8 ± 3 | 5.6 ± 3/9 ± 1.7 | 6 ± 3.5/9 ± 1 | ||
| CN | 4.8 ± 1/6 ± 2 | 88 ± 4/90 ± 48 | 9 ± 7/7 ± 2.8 | 12 ± 5.5/8 ± 2.7 | 7 ± 5/6.6 ± 3 | 10 ± 7/7.3 ± 3 | 6.4 ± 5/8.3 ± 1 | 6 ± 1.8/7 ± 0.2 | ||
| STR-12 | 575 ± 27/385 ± 16 | NT | 2 ± 0.8/4 ± 1 | 96 ± 4/63 ± 4 | 97 ± 1/56 ± 19 | 7 ± 6/4.7 ± 1 | 5 ± 3/4 ± 0.5 | 3.7 ± 2/4 ± 0.5 | 4 ± 2.5/4.3 ± 1 | 2.7 ± 1.4/4 ± 1 |
| NC | 2 ± 0.1/4 ± 0.6 | 95 ± 6/49 ± 3 | 96 ± 1/57 ± 6 | 4 ± 3/4 ± 0.4 | 4 ± 2.6/4 ± 0.1 | 3 ± 1.4/4 ± 0.4 | 3 ± 1/4 ± 0.3 | 3 ± 0.4/3 ± 0.5 | ||
| CN | 3 ± 0.8/3 ± 0.3 | 98 ± 2/50 ± 5 | 97 ± 1/48 ± 14 | 12 ± 9/4.4 ± 0.7 | 7 ± 3.5/4 ± 0.4 | 4 ± 0.8/4 ± 0.4 | 4.5 ± 1/4 ± 0.5 | 4 ± 2/4 ± 0.6 |
| Cell population . | FSC/SSC . | Treatment . | Control . | CD44 . | VCAM . | α4 . | β2 . | β7 . | P-selectin . | L-selectin . |
|---|---|---|---|---|---|---|---|---|---|---|
| HUVECs | 369 ± 23/414 ± 12 | NT | 5.7 ± 2/5.3 ± 2 | 95 ± 4/167 ± 63 | 8 ± 3/6 ± 2 | 12 ± 3.4/7 ± 2.3 | 5.7 ± 5.3/7 ± 1 | 8.4 ± 5/7 ± 1.4 | 5.7 ± 1/7 ± 0.3 | 7 ± 4/7 ± 0.7 |
| NC | 4 ± 2/6.7 ± 2.6 | 82 ± 17/79 ± 47 | 7 ± 3/7.5 ± 2.5 | 9.5 ± 2/8.5 ± 3 | 7 ± 4/7 ± 2.6 | 8.6 ± 7/7.8 ± 3 | 5.6 ± 3/9 ± 1.7 | 6 ± 3.5/9 ± 1 | ||
| CN | 4.8 ± 1/6 ± 2 | 88 ± 4/90 ± 48 | 9 ± 7/7 ± 2.8 | 12 ± 5.5/8 ± 2.7 | 7 ± 5/6.6 ± 3 | 10 ± 7/7.3 ± 3 | 6.4 ± 5/8.3 ± 1 | 6 ± 1.8/7 ± 0.2 | ||
| STR-12 | 575 ± 27/385 ± 16 | NT | 2 ± 0.8/4 ± 1 | 96 ± 4/63 ± 4 | 97 ± 1/56 ± 19 | 7 ± 6/4.7 ± 1 | 5 ± 3/4 ± 0.5 | 3.7 ± 2/4 ± 0.5 | 4 ± 2.5/4.3 ± 1 | 2.7 ± 1.4/4 ± 1 |
| NC | 2 ± 0.1/4 ± 0.6 | 95 ± 6/49 ± 3 | 96 ± 1/57 ± 6 | 4 ± 3/4 ± 0.4 | 4 ± 2.6/4 ± 0.1 | 3 ± 1.4/4 ± 0.4 | 3 ± 1/4 ± 0.3 | 3 ± 0.4/3 ± 0.5 | ||
| CN | 3 ± 0.8/3 ± 0.3 | 98 ± 2/50 ± 5 | 97 ± 1/48 ± 14 | 12 ± 9/4.4 ± 0.7 | 7 ± 3.5/4 ± 0.4 | 4 ± 0.8/4 ± 0.4 | 4.5 ± 1/4 ± 0.5 | 4 ± 2/4 ± 0.6 |
Cells were not treated or were treated with 10−4 M (for 2 hours) nicotine or cotinine. Expression of adhesion molecules was evaluated by FACS and expressed as means ± SD of the percentage of the gated cell population/mean fluorescence intensity. Data from 2 to 5 independent experiments are presented.
FSC indicates forward light scatter; SSC, side light scatter; VCAM, vascular cell adhesion molecule; HUVECs, human umbilical vein endothelial cells; NT, no treatment; NC, nicotine; CN, cotinine; STR-12, murine bone marrow–derived endothelial cell line.