Effect of interleukin-3 and stem cell factor on expression of RCAS1-receptor
| Culture conditions . | Day 8 ECFCs . |
|---|---|
| EPO alone | 100.0% ± 21.1% |
| EPO + IL-3 | 112.4% ± 12.9% |
| EPO + SCF | 264.7% ± 127.7%* |
| EPO + IL-3 + SCF | 265.4% ± 118.2%† |
| Culture conditions . | Day 8 ECFCs . |
|---|---|
| EPO alone | 100.0% ± 21.1% |
| EPO + IL-3 | 112.4% ± 12.9% |
| EPO + SCF | 264.7% ± 127.7%* |
| EPO + IL-3 + SCF | 265.4% ± 118.2%† |
To determine the effect of IL-3 and SCF on the expression of RCAS1R, 3 independent experiments were done, using ECFCs purified from 3 donors. Day 6 ECFCs (1 × 105 cells/mL) were incubated in serum-free condition media containing 10 U/mL rhEPO at 37°C in a high-humidity 5% CO2, 95% air incubator. Then 10 ng/mL rhIL-3 and 100 ng/mL rhSCF were added, as indicated. After 48 hours, the expression of RCAS1R on the cell surface was detected, using flow cytometry. Values are expressed as the percentage against the mean of EPO alone, and are mean ± SD from triplicate determinations.
EPO indicates erythropoietin; IL-3, interleukin-3; SCF, stem cell factor.
Versus EPO alone, P = .016, versus EPO + IL-3, P = .022.
Versus EPO alone, P = .015, versus EPO + IL-3, P = .021.