In vitro functional activity of monoclonal anticardiolipin Immunoglobulin G (IgG) and control IgG
| . | Control IgG . | CIC 01 . | CIC 03 . | CIC 11 . | CIC 14 . | CIC 19 . |
|---|---|---|---|---|---|---|
| LA activity using the dRVVT test | ||||||
| Clotting time(s) dRVVT* | 43.5 ± 0.7 | 60.1 ± 0.8 | 45.85 ± 1.35 | 45.25 ± 0.45 | 45 ± 0.8 | 45.1 ± 1.2 |
| Confirmatory test | NA | 46.4 ± 0.6 | NA | NA | NA | NA |
| Ratio† | — | 1.29 | — | — | — | — |
| Percentage of inhibition of thrombin generation | ||||||
| mAb concentration | ||||||
| 1 μg/mL | 0 | 0 | 12 | 0 | 14 | 5 |
| 5 μg/mL | 0 | 15 | 20 | 0 | 16 | 12 |
| 10 μg/mL | 0 | 32 | 45 | 0 | 20 | 16 |
| 20 μg/mL | 0 | 58 | ND | ND | 18 | 18 |
| . | Control IgG . | CIC 01 . | CIC 03 . | CIC 11 . | CIC 14 . | CIC 19 . |
|---|---|---|---|---|---|---|
| LA activity using the dRVVT test | ||||||
| Clotting time(s) dRVVT* | 43.5 ± 0.7 | 60.1 ± 0.8 | 45.85 ± 1.35 | 45.25 ± 0.45 | 45 ± 0.8 | 45.1 ± 1.2 |
| Confirmatory test | NA | 46.4 ± 0.6 | NA | NA | NA | NA |
| Ratio† | — | 1.29 | — | — | — | — |
| Percentage of inhibition of thrombin generation | ||||||
| mAb concentration | ||||||
| 1 μg/mL | 0 | 0 | 12 | 0 | 14 | 5 |
| 5 μg/mL | 0 | 15 | 20 | 0 | 16 | 12 |
| 10 μg/mL | 0 | 32 | 45 | 0 | 20 | 16 |
| 20 μg/mL | 0 | 58 | ND | ND | 18 | 18 |
LA indicates lupus anticoagulant; dRVVT, dilute Russell's viper venom test; mAb, monoclonal antibodies; NA, not applicable, because only the samples significantly prolonging the dRVVT required a confirmatory test (see “Patient, materials, and methods”); ND, not done.
Each figure in the row represents an average of 2 tests.
The clotting times of dRVVT/dRVVT confirmatory test.