Molecular analysis of the V regions of the H and L chains of the monoclonal anticardiolipin Immunoglobulin G
| mAb . | VHfamily . | Germline % homology . | R/S CDR . | R/S FR . | D gene . | JH gene . | Vκ family . | Germline % homology . | R/S CDR . | R/S FR . | Jκ gene . |
|---|---|---|---|---|---|---|---|---|---|---|---|
| CIC 01 | 5 | V5-51*02 | 2.75 | 3.6 | D5-24*01/in | 5 | 1 | V1-9*01 | 0.9 | 1 | |
| 82.1% | P < .05 | D6-13*01/in | 88% | P > .05 | |||||||
| CIC 03 | 4 | V4-4*05 | 1.7 | 2 | D1-1*01/in | 3 | 1 | V1-5*03 | 2 | 1.37 | 1 |
| 91.7% | P > .05 | D5-5*01 | 91.6% | P > .05 | |||||||
| CIC 11 | 3 | V3-48*02 | 8 | 1 | D2-21*01/in | 4 | 1 | V1-9*01 | — | 4 | 5 |
| 93.8% | P < .05 | 97.4% | |||||||||
| CIC 14 | 3 | V3-13*01 | D3-10*02 | 6 | 1 | V1-9*01 | 1 | 3 | |||
| 97.3% | P > .05 | 98.5% | P < .05 | ||||||||
| CIC 19 | 3 | V3-30*02 | — | — | D3-16*01 | 4 | 3 | V3-20*01 | — | 1 | |
| 100% | 99.6% |
| mAb . | VHfamily . | Germline % homology . | R/S CDR . | R/S FR . | D gene . | JH gene . | Vκ family . | Germline % homology . | R/S CDR . | R/S FR . | Jκ gene . |
|---|---|---|---|---|---|---|---|---|---|---|---|
| CIC 01 | 5 | V5-51*02 | 2.75 | 3.6 | D5-24*01/in | 5 | 1 | V1-9*01 | 0.9 | 1 | |
| 82.1% | P < .05 | D6-13*01/in | 88% | P > .05 | |||||||
| CIC 03 | 4 | V4-4*05 | 1.7 | 2 | D1-1*01/in | 3 | 1 | V1-5*03 | 2 | 1.37 | 1 |
| 91.7% | P > .05 | D5-5*01 | 91.6% | P > .05 | |||||||
| CIC 11 | 3 | V3-48*02 | 8 | 1 | D2-21*01/in | 4 | 1 | V1-9*01 | — | 4 | 5 |
| 93.8% | P < .05 | 97.4% | |||||||||
| CIC 14 | 3 | V3-13*01 | D3-10*02 | 6 | 1 | V1-9*01 | 1 | 3 | |||
| 97.3% | P > .05 | 98.5% | P < .05 | ||||||||
| CIC 19 | 3 | V3-30*02 | — | — | D3-16*01 | 4 | 3 | V3-20*01 | — | 1 | |
| 100% | 99.6% |
“Germline % homology” provides the percentage of nucleotide sequence homology between the Ig V gene and its putative germline. P is the probability that an excess of R mutations in the CDRs resulted by chance.50
R/S indicates the ratio of replacement mutations (R) and silent mutations (S) in the complementary determining regions (CDRs [CDR1 and CDR2]) and framework (FR1, FR2, and FR3) regions; in, sequences inserted in inverted orientation. See Table 1 for other abbreviations.