Method for calculation of ratio of actual to expected engraftment
| Step . | Calculation . | Result . |
|---|---|---|
| 1 | No. adherent layer cells harvested from 25-cm2 flask | Flask yield |
| 2 | No. adherent layer cells injected ÷ flask yield | Flask equivalent injected |
| 3 | No. fresh male cells inoculated onto 25-cm2 flasks initially × flask equivalent injected | Male input equivalent injected |
| 4 | (No. adherent layer cells injected × % female in adherent layer [by FISH]) + no. fresh female cells injected | Total female competition injected |
| 5 | Male input equivalent injected ÷ (male input equivalent injected + total female competition injected) | Expected engraftment |
| 6 | Actual % male chimerism for each recipient ÷ expected engraftment | Ratio of actual to expected engraftment |
| Step . | Calculation . | Result . |
|---|---|---|
| 1 | No. adherent layer cells harvested from 25-cm2 flask | Flask yield |
| 2 | No. adherent layer cells injected ÷ flask yield | Flask equivalent injected |
| 3 | No. fresh male cells inoculated onto 25-cm2 flasks initially × flask equivalent injected | Male input equivalent injected |
| 4 | (No. adherent layer cells injected × % female in adherent layer [by FISH]) + no. fresh female cells injected | Total female competition injected |
| 5 | Male input equivalent injected ÷ (male input equivalent injected + total female competition injected) | Expected engraftment |
| 6 | Actual % male chimerism for each recipient ÷ expected engraftment | Ratio of actual to expected engraftment |
FISH indicates fluorescence in situ hybridization.