Comparison of cell numbers injected and actual chimerism results to ratio of actual to expected engraftment
| Adhesion time . | Adherent layer cells injected (× 106) . | Ratio of adherent layer cells to fresh female cells* . | Flask equivalent injected . | Ratio of input male equivalent cells to total female cells† . | Actual % male chimerism‡ . | Ratio of actual to expected engraftment‡ . |
|---|---|---|---|---|---|---|
| Short engraftment time | ||||||
| 20 minutes | 15.0 | 15 | 1.8 | 2.5 | 4.5 ± 0.5 | 6.3 ± 0.7 |
| 40 minutes | 17.8 | 17.8 | 1.8 | 2.5 | 10.8 ± 3.3 | 15.2 ± 4.7 |
| 1 hour | 19.5 | 19.5 | 1.8 | 2.4 | 18.5 ± 3.2 | 26.2 ± 4.5 |
| Long engraftment time | ||||||
| 20 minutes | 15.0 | 15 | 1.8 | 2.5 | 10.9 ± 3.3 | 15.2 ± 4.6 |
| 40 minutes | 17.8 | 17.8 | 1.8 | 2.5 | 17.2 ± 2.3 | 24.2 ± 3.2 |
| 1 hour | 12.3 | 17.3 | 1.7 | 6.0 | 63.7 ± 3.0 | 79.4 ± 6.9 |
| 6 hours | 6.7 | 16 | 1.7 | 8.6 | 51.7 ± 2.5 | 57.7 ± 2.7 |
| 24 hours | 4.6 | 15.9 | 1.7 | 8.8 | 59.5 ± 4.1 | 66.3 ± 4.6 |
| Adhesion time . | Adherent layer cells injected (× 106) . | Ratio of adherent layer cells to fresh female cells* . | Flask equivalent injected . | Ratio of input male equivalent cells to total female cells† . | Actual % male chimerism‡ . | Ratio of actual to expected engraftment‡ . |
|---|---|---|---|---|---|---|
| Short engraftment time | ||||||
| 20 minutes | 15.0 | 15 | 1.8 | 2.5 | 4.5 ± 0.5 | 6.3 ± 0.7 |
| 40 minutes | 17.8 | 17.8 | 1.8 | 2.5 | 10.8 ± 3.3 | 15.2 ± 4.7 |
| 1 hour | 19.5 | 19.5 | 1.8 | 2.4 | 18.5 ± 3.2 | 26.2 ± 4.5 |
| Long engraftment time | ||||||
| 20 minutes | 15.0 | 15 | 1.8 | 2.5 | 10.9 ± 3.3 | 15.2 ± 4.6 |
| 40 minutes | 17.8 | 17.8 | 1.8 | 2.5 | 17.2 ± 2.3 | 24.2 ± 3.2 |
| 1 hour | 12.3 | 17.3 | 1.7 | 6.0 | 63.7 ± 3.0 | 79.4 ± 6.9 |
| 6 hours | 6.7 | 16 | 1.7 | 8.6 | 51.7 ± 2.5 | 57.7 ± 2.7 |
| 24 hours | 4.6 | 15.9 | 1.7 | 8.8 | 59.5 ± 4.1 | 66.3 ± 4.6 |
Results of 4 experiments (3 to 5 recipients per group per experiment) are shown combined. Short engraftment is 2 weeks; long engraftment is 8 to 11 weeks.
Ratio of the number of adherent layer cells to fresh female cells injected (calculated at the time of transplantation).
Ratio of the number of input male equivalent cells to total female cells injected (calculated retrospectively by means of fluorescence in situ hybridization results of adherent layer female percentage).
Mean percentage ± SEM.