Frequency of cultures with natural killer cell growth and receptor expression
| FL, KL, IL-3+ . | NK growth* . | CD94+ NK cells† . | KIR+ NK cells† . | |||
|---|---|---|---|---|---|---|
| Day 25 (25-100 starting cells) (%) . | Day 50 (10-25 starting cells) (%) . | Day 25 (%) . | Day 50 (%) . | Day 25 (%) . | Day 50 (%) . | |
| None | 43/46 (93) | 9/32 (28) | 14 | ND | 0 | ND |
| IL-7 | 45/46 (98) | 28/32 (88) | 75 | 89 | 48 | 18 |
| IL-15 | 29/29 (100) | 28/32 (88) | 100 | 96 | 72 | 89 |
| IL-7 + IL-15 | 29/29 (100) | 26/28 (93) | 100 | 96 | 76 | 85 |
| IL-2 | 45/45 (100) | 35/40 (88) | 100 | 95 | 64 | 91 |
| IL-7 + IL-2 | 46/46 (100) | 29/40 (98) | 98 | 92 | 72 | 82 |
| FL, KL, IL-3+ . | NK growth* . | CD94+ NK cells† . | KIR+ NK cells† . | |||
|---|---|---|---|---|---|---|
| Day 25 (25-100 starting cells) (%) . | Day 50 (10-25 starting cells) (%) . | Day 25 (%) . | Day 50 (%) . | Day 25 (%) . | Day 50 (%) . | |
| None | 43/46 (93) | 9/32 (28) | 14 | ND | 0 | ND |
| IL-7 | 45/46 (98) | 28/32 (88) | 75 | 89 | 48 | 18 |
| IL-15 | 29/29 (100) | 28/32 (88) | 100 | 96 | 72 | 89 |
| IL-7 + IL-15 | 29/29 (100) | 26/28 (93) | 100 | 96 | 76 | 85 |
| IL-2 | 45/45 (100) | 35/40 (88) | 100 | 95 | 64 | 91 |
| IL-7 + IL-2 | 46/46 (100) | 29/40 (98) | 98 | 92 | 72 | 82 |
All cultures were initiated with CD34+/Lin−/CD38− UCB cells, AFT024, FL, KL, IL-3, and the indicated cytokines (n = 3-5 individual UCB for each condition with multiple replicates).
NK indicates natural killer; KIR, killer immuoglobulinlike receptor; FL, Flt3 ligand; KL, c-kit ligand; IL, interleukin; ND, not done; UCB, umbilical cord blood.
The percentage with NK growth is the number of wells with CD56+ cells/replicate wells plated.
The percentage with CD94 or KIR expression is the positive/number of wells with NK cells.