Frequency of single cells with natural killer cell progeny expressing CD94 or killer immunoglobulinlike receptor
| FL, KL, IL-3+ . | n . | Cloning frequency (%) . | CD94 (%) . | KIR3DL1 (%) . | KIR2DL1/S1 (%) . | KIR2DL2/L3/S2 (%) . |
|---|---|---|---|---|---|---|
| IL-7 | 4 | 7 | 5/12 (42) | 8/32 (25) | 5/19 (26) | 6/19 (32) |
| IL-7 + IL-15 | 10 | 22 | 46/46 (100) | 100/196 (51) | 80/153 (52) | 108/152 (72) |
| IL-7 + IL-2 | 11 | 19 | 38/41 (93) | 144/252 (57) | 141/197 (71) | 155/198 (78) |
| FL, KL, IL-3+ . | n . | Cloning frequency (%) . | CD94 (%) . | KIR3DL1 (%) . | KIR2DL1/S1 (%) . | KIR2DL2/L3/S2 (%) . |
|---|---|---|---|---|---|---|
| IL-7 | 4 | 7 | 5/12 (42) | 8/32 (25) | 5/19 (26) | 6/19 (32) |
| IL-7 + IL-15 | 10 | 22 | 46/46 (100) | 100/196 (51) | 80/153 (52) | 108/152 (72) |
| IL-7 + IL-2 | 11 | 19 | 38/41 (93) | 144/252 (57) | 141/197 (71) | 155/198 (78) |
All cultures were initiated with UCB CD34+Lin−CD38− cells, AFT024, FL, KL, IL-3, and the indicated cytokines (n = the number of individual UCB). Results are from cultures harvested after 26-36 days. The cloning frequency is the number of wells with NK-cell progency divided by the total number of single cells plated. For CD94 and KIR expression, the denominator is the total number of NK-cell populations evaluated.
For abbreviations, see Table 2.