The HLA type of the progenitor source does not determine killer immunoglobulinlike receptor expression
| HLA allele4-150 . | KIR mab4-151 . | NK progeny with KIR from HSC with allele‡ (%) . | NK progeny with KIR from HSC without allele‡ (%) . | Absolute number KIR+ NK from HSC with allele4-153 . | Absolute number KIR+ NK from HSC without allele4-153 . |
|---|---|---|---|---|---|
| Bw4 | DX9 | 12/41 (29) | 22/80 (28) | 874 ± 287 | 567 ± 86 |
| Cw4 | HP-3E4 | 12/36 (33) | 57/134 (43) | 488 ± 108 | 1180 ± 197 |
| Cw3 | GL183 | 94/103 (75) | 33/46 (72) | 9671 ± 1929 | 7010 ± 2773 |
| HLA allele4-150 . | KIR mab4-151 . | NK progeny with KIR from HSC with allele‡ (%) . | NK progeny with KIR from HSC without allele‡ (%) . | Absolute number KIR+ NK from HSC with allele4-153 . | Absolute number KIR+ NK from HSC without allele4-153 . |
|---|---|---|---|---|---|
| Bw4 | DX9 | 12/41 (29) | 22/80 (28) | 874 ± 287 | 567 ± 86 |
| Cw4 | HP-3E4 | 12/36 (33) | 57/134 (43) | 488 ± 108 | 1180 ± 197 |
| Cw3 | GL183 | 94/103 (75) | 33/46 (72) | 9671 ± 1929 | 7010 ± 2773 |
mab indicates monoclonal antibody; HSC, hematopoietic stem cell; MHC, major histocompatibility complex; for other abbreviations, see Table 2.
Based on serologic HLA typing of 6 UCB units.
Monoclonal antibody for KIR that recognizes respective class I MHC allele.
Single CD34+/Lin−/CD38− HSCs were sorted from UCB units and data are grouped based on the HLA type of the HSC source. All cultures were initiated with single cells and contained AFT024, FL, KL, IL-3, IL-7, and IL-2. Shown is the number of single-cell NK-cell progeny expressing the KIR that recognize the respective class I ligand (number positive/number analyzed [percent positive]) based on the HSC source with or without the class I MHC KIR ligand of interest (Bw4, Cw4 group, Cw3 group).
The absolute number of NK cells derived from a single HSC expressing the KIR that recognizes the respective class I ligand was calculated. The numerator of the respective previous columns is the number of replicates that contributed to each absolute NK cell number.