Effect of interleukin-12 administration on the induction of immunological tolerance by UV-B–irradiated allogeneic leukocytes
| . | Anti-H-2dantibody activity* (fluorescent intensity, mean ± SD, n = 4) . |
|---|---|
| Naive Control | 212 ± 22 |
| UVB-MNL | 12 ± 2 |
| UVB-MNL + IL-12 | 23 ± 6 |
| Preimmune Sera | 12 ± 1 |
| . | Anti-H-2dantibody activity* (fluorescent intensity, mean ± SD, n = 4) . |
|---|---|
| Naive Control | 212 ± 22 |
| UVB-MNL | 12 ± 2 |
| UVB-MNL + IL-12 | 23 ± 6 |
| Preimmune Sera | 12 ± 1 |
UVB-MNL are CBA mice received 4 weekly transfusions of UVB-irradiate MNL from BALB/c mice for induction of tolerance.
UVB-MNL + IL-12 are CBA mice received the same 4 transfusions of UVB-MNL plus intraperitoneal injection of 500 ng IL-12.
MNL indicates mononuclear leukocytes. Other abbreviations are explained in Table 1.
After 4 weekly transfusion of UVB-MNL, all CBA mice including the naive controls were challenged with 2 weekly transfusions of a fully immunogenic dose of 2 × 105 BALB/c MNL. One week later, all mice were bled for testing of anti-H-2dantibody activity. The mice treated with UVB-MNL + IL-12 developed low levels of anti-H-2d antibody.