Antibody panel used
| CD/FITC, PE, PerCP . | Cell population defined . |
|---|---|
| CD45/PerCP | All hematopoietic cells |
| CD14/PE | Monocytes |
| CD61/FITC | Megakaryocytes |
| CD41/FITC | Magakaryocytes |
| CD3/FITC, PE | Mature T cells |
| CD8/FITC, PE | T cells, NK cells |
| CD4/FITC, PE | T cells |
| CD2/FITC | T cells |
| CD5/FITC | T cells |
| Anti–glycophorin A/PE | Erythroid lineage |
| CD71/FITC | Transferrin receptor—different expression in multiple lineages |
| CD11b/PE | Mature and intermediate differentiated myeloid series |
| CD19/FITC | B cells |
| CD20/PE | B cells |
| CD22/PE | B cells |
| CD16/PE | T cells, NK cells, and myeloid lineage |
| CD57/FITC | T cells, NK cells |
| CD56/PE | T cells, NK cells |
| CD64/PE | Myeloid cells, mature granulocytes, and monocytes |
| Anti-κ/FITC | B cells |
| Anti-λ/PE | B cells |
| CD36/FITC | Monocytic, erythroid, and megakaryocytic lineages |
| CD7/PE | T cells |
| CD10/PE | Pre-B cells, mature granulocytes |
| CD/FITC, PE, PerCP . | Cell population defined . |
|---|---|
| CD45/PerCP | All hematopoietic cells |
| CD14/PE | Monocytes |
| CD61/FITC | Megakaryocytes |
| CD41/FITC | Magakaryocytes |
| CD3/FITC, PE | Mature T cells |
| CD8/FITC, PE | T cells, NK cells |
| CD4/FITC, PE | T cells |
| CD2/FITC | T cells |
| CD5/FITC | T cells |
| Anti–glycophorin A/PE | Erythroid lineage |
| CD71/FITC | Transferrin receptor—different expression in multiple lineages |
| CD11b/PE | Mature and intermediate differentiated myeloid series |
| CD19/FITC | B cells |
| CD20/PE | B cells |
| CD22/PE | B cells |
| CD16/PE | T cells, NK cells, and myeloid lineage |
| CD57/FITC | T cells, NK cells |
| CD56/PE | T cells, NK cells |
| CD64/PE | Myeloid cells, mature granulocytes, and monocytes |
| Anti-κ/FITC | B cells |
| Anti-λ/PE | B cells |
| CD36/FITC | Monocytic, erythroid, and megakaryocytic lineages |
| CD7/PE | T cells |
| CD10/PE | Pre-B cells, mature granulocytes |
All antibodies were obtained from Becton Dickinson, with the exception of CD7, which was obtained from Coulter Immunology, Hialeah, FL.
FITC indicates fluorescein isothiocyanate; PE, phycoerythrin; PerCP, peridinin chlorophyll protein; NK, natural killer.