ATM primers for reverse transcription-polymerase chain reaction, restriction endonuclease fingerprinting analysis, and cDNA sequencing
| ATM primers for RT-PCR and REF . | ATM primers for cDNA sequencing . |
|---|---|
| Fragment 1104 bp 5′ GTGTTCTGAAATTGTGAACCATGAGTCTAGT 5′ TGGTATCTTCATTAAAAACCTGGTGACAGA Fragment 1629 bp 5′ AGTAGAGGAAAGTATTCTTCAGGATTTCG 5′ CGTTTGCATCAGTAACACTACTATCAG Fragment 1608 bp 5′ GAGGTGGAGGATCAGTCATCCATGAATC 5′ GCGATGGAAAATAGGTGGATTAGGAGCAG Fragment 1121 bp 5′ CAGAGATTGTGGTGGAGTTAGTTATTG 5′ GCATTATGAAGGTCCACTGAAG Fragment 1058 bp 5′ TCTAGAGGCTGTTGGAAGCTGCT 5′ CCATACAAACTATCTGGCTCC Fragment 1233 bp 5′ CTGGAATAAGTTTACAGGATCTTC 5′ GATGATTTCATGTAGTTTTCAATTC Fragment 932 bp 5′ GATGGAGAAAGTAGTGATGAGC 5′ AGTCACCAGATTTCCATATTCTC Fragment 1227 bp 5′ AAGATGTTGTTGTCCCTACTATG 5′ AAGGCTGAATGAAAGGGTAATTC | Fragment 1 (2882 bp) 5′ GTGTTCTGAAATTGTGAACCATGAGTCTAG 5′ TATATGACTTATCTCATTCACTAGCAGATC 5′ TGAATTAGGAGATGAAATTCTTCCCAC 5′ TGACTTTGTCACTGACCACCAGTATAGTTC 5′ CTGGGATTATCAGAACAGCTTCTG 5′ TCCTGCGATTGTTAACATCAAAG 5′AAGCTCCTTTAAAAGCATTAGATACATATG Fragment 2 (2489 bp) 5′ GGCTTTTTCCTGCGATTGTTAACATCAAAGC 5′ AACTACTGCTCAGACCAATACTGTG 5′ GCTGACAATCATCACCAAGTTCG 5′ TTCTTGTAAATATTCTTCCTTATTTTGCCT 5′ ACCTCATTTTCCATCGCATGTGATTAA 5′ GTCCACCATCTGATCTTTATGTAGTTCCAGTTG Fragment 3 (2649 bp) 5′ TGAAAACCTCTATATCACGATTAAGC 5′ TCTAGAGGCTGTTGGAAGCTGCT 5′ GATTCATGATATTTTAGTCCAAGATAC 5′ CTGGAATAAGTTTACAGGATCTTC 5′ GACCATTGCACTTCCGTCAGC 5′ AAACACTCCCAGCTTCTCAAGG 5′ GTTTGTCTGAATTTTATGTTCCCTAAG Fragment 4 (2232 bp) 5′ CAATCCCAGCCTAAAACTTACATACACAGAAT 5′ GTCAATGGCATGATGAAGAGAGACGGA 5′ CAGTGCCAAAAGAAAATGATGGAGGT 5′ TAGCTGGATCCAAGAATTTTTCC 5′ TCTAAAGGCTGAATGAAAGGGTAATTC |
| ATM primers for RT-PCR and REF . | ATM primers for cDNA sequencing . |
|---|---|
| Fragment 1104 bp 5′ GTGTTCTGAAATTGTGAACCATGAGTCTAGT 5′ TGGTATCTTCATTAAAAACCTGGTGACAGA Fragment 1629 bp 5′ AGTAGAGGAAAGTATTCTTCAGGATTTCG 5′ CGTTTGCATCAGTAACACTACTATCAG Fragment 1608 bp 5′ GAGGTGGAGGATCAGTCATCCATGAATC 5′ GCGATGGAAAATAGGTGGATTAGGAGCAG Fragment 1121 bp 5′ CAGAGATTGTGGTGGAGTTAGTTATTG 5′ GCATTATGAAGGTCCACTGAAG Fragment 1058 bp 5′ TCTAGAGGCTGTTGGAAGCTGCT 5′ CCATACAAACTATCTGGCTCC Fragment 1233 bp 5′ CTGGAATAAGTTTACAGGATCTTC 5′ GATGATTTCATGTAGTTTTCAATTC Fragment 932 bp 5′ GATGGAGAAAGTAGTGATGAGC 5′ AGTCACCAGATTTCCATATTCTC Fragment 1227 bp 5′ AAGATGTTGTTGTCCCTACTATG 5′ AAGGCTGAATGAAAGGGTAATTC | Fragment 1 (2882 bp) 5′ GTGTTCTGAAATTGTGAACCATGAGTCTAG 5′ TATATGACTTATCTCATTCACTAGCAGATC 5′ TGAATTAGGAGATGAAATTCTTCCCAC 5′ TGACTTTGTCACTGACCACCAGTATAGTTC 5′ CTGGGATTATCAGAACAGCTTCTG 5′ TCCTGCGATTGTTAACATCAAAG 5′AAGCTCCTTTAAAAGCATTAGATACATATG Fragment 2 (2489 bp) 5′ GGCTTTTTCCTGCGATTGTTAACATCAAAGC 5′ AACTACTGCTCAGACCAATACTGTG 5′ GCTGACAATCATCACCAAGTTCG 5′ TTCTTGTAAATATTCTTCCTTATTTTGCCT 5′ ACCTCATTTTCCATCGCATGTGATTAA 5′ GTCCACCATCTGATCTTTATGTAGTTCCAGTTG Fragment 3 (2649 bp) 5′ TGAAAACCTCTATATCACGATTAAGC 5′ TCTAGAGGCTGTTGGAAGCTGCT 5′ GATTCATGATATTTTAGTCCAAGATAC 5′ CTGGAATAAGTTTACAGGATCTTC 5′ GACCATTGCACTTCCGTCAGC 5′ AAACACTCCCAGCTTCTCAAGG 5′ GTTTGTCTGAATTTTATGTTCCCTAAG Fragment 4 (2232 bp) 5′ CAATCCCAGCCTAAAACTTACATACACAGAAT 5′ GTCAATGGCATGATGAAGAGAGACGGA 5′ CAGTGCCAAAAGAAAATGATGGAGGT 5′ TAGCTGGATCCAAGAATTTTTCC 5′ TCTAAAGGCTGAATGAAAGGGTAATTC |
The left-hand panel shows ATM primers for amplification of 8 overlapping fragments covering the entireATM cDNA from the 5′ to 3′ end, used for REF. The right-hand panel shows primers used for cDNA sequencing. Primers in italics were also used for amplification of 4 overlapping ATM fragments, from which sequencing was carried out.