Sequence of oligomers used for reverse transcriptase–polymerase chain reaction
| PCR target . | Primer . | Sequence . | Reference . |
|---|---|---|---|
| NPM-ALK | NPM forward | 5-TCCCTTGGGGGCTTTGAAATAACACC | Morris et al3 |
| ALK reverse | 5-CGAGGTGCGGAGCTTGCTCAGC | ||
| TPM3-ALK | C-TPM1 (outer) | 5-CGAGAAGTTGAGGGAGAAAGG | Lamant et al5 |
| ALK-1 (outer) | 5-GCCAGCAAAGCAGTAGTTGGGGTTG | ||
| C-TPM3 (inner) | 5-CTGGCAGAGTCCCGTTGCC | ||
| ALK-2 (inner) | 5-GTCGAGGTGCGGAGCTTGCTCAGC | ||
| ATIC-ALK | ATIC (outer) | 5-CGCTCGCCCTGAACCCAGTG | Ma et al6 |
| ATIC (inner) | 5-GTGTCCACGGAGATGCAGAG | ||
| ALK (outer and inner) | 5-CGAGGTGCGGAGCTTGCTCAGC | ||
| TPM4-ALK | TPM4Start | 5-CGCGCCATGGCCGGCCTCAAC | This report |
| ALKSM3R | 5-AGCACACTTCAGGCAGCGTCTTC | ||
| ABL | ABLX3 | 5-TTTCTCCAGACTGTTGACTGG | Hunger et al48 |
| ABLX2 | 5-CCTTCAGCGGCCAGTAGCAT |
| PCR target . | Primer . | Sequence . | Reference . |
|---|---|---|---|
| NPM-ALK | NPM forward | 5-TCCCTTGGGGGCTTTGAAATAACACC | Morris et al3 |
| ALK reverse | 5-CGAGGTGCGGAGCTTGCTCAGC | ||
| TPM3-ALK | C-TPM1 (outer) | 5-CGAGAAGTTGAGGGAGAAAGG | Lamant et al5 |
| ALK-1 (outer) | 5-GCCAGCAAAGCAGTAGTTGGGGTTG | ||
| C-TPM3 (inner) | 5-CTGGCAGAGTCCCGTTGCC | ||
| ALK-2 (inner) | 5-GTCGAGGTGCGGAGCTTGCTCAGC | ||
| ATIC-ALK | ATIC (outer) | 5-CGCTCGCCCTGAACCCAGTG | Ma et al6 |
| ATIC (inner) | 5-GTGTCCACGGAGATGCAGAG | ||
| ALK (outer and inner) | 5-CGAGGTGCGGAGCTTGCTCAGC | ||
| TPM4-ALK | TPM4Start | 5-CGCGCCATGGCCGGCCTCAAC | This report |
| ALKSM3R | 5-AGCACACTTCAGGCAGCGTCTTC | ||
| ABL | ABLX3 | 5-TTTCTCCAGACTGTTGACTGG | Hunger et al48 |
| ABLX2 | 5-CCTTCAGCGGCCAGTAGCAT |
PCR indicates polymerase chain reaction; NPM, nucleophosmin; ALK, anaplastic lymphoma kinase; TPM, tropomyosin; ATIC, 5-aminoimadazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase; ABL, Abelson leukemia gene.