Reverse transcription–polymerase chain reaction primers and conditions
| cDNA . | Length . | Tm . | Cycles . | 5′ sequence . | 3′ sequence . | Reference . |
|---|---|---|---|---|---|---|
| GATA-2 | 720 | 55 | 32 | 5′ CGG AAT TCG ACA CAC CAC CCG ATA CCC ACC TAT 3′ | 5′ CGG AAT TCG CCT ACG CCA TGG CAG TCA CCA TGC T 3′ | 67 |
| c-mpl | 322 | 60 | 30 | 5′ GCA GCA CCC AGT GGG ACA TAC CAG 3′ | 5′ GCC ATA GCG GAG TTC ATG CCT CAG G 3′ | 65 |
| NF-E2 | 400 | 60 | 34 | 5′ AGA TGG AAC TGA CTT GGC AAG 3′ | 5′ TCT GCA TCA CTG TAG TTG AGG 3′ | 66 |
| GATA-1 | 375 | 60 | 32 | 5′ GGA ATT CGG GCC CCT TGT GAG GCC AGA GAG 3′ | 5′ CGG GGT ACC TCA CGC TCC AGC CAG ATT CGA CCC 3′ | 68 |
| EPO-R | 452 | 55 | 32 | 5′ GGA CAC CTA CTT GGT ATT GG 3′ | 5′ GAC GTT GTA GGC TGG AGT CC 3′ | 69 |
| C/EBPα | 458 | 55 | 32 | 5′ TTC TAC GAG GCG GAG CCG CGG 3′ | 5′ GAT CAC CAG CGG CCG CAG CGC 3′ | 70 |
| Pax-5 | 439 | 65 | 36 | 5′ CTA CAG GCT CCG TGA CGC AG 3′ | 5′ TCT CGG CCT GTG ACA ATA GG 3′ | 72 |
| Aiolos | 463 | 65 | 32 | 5′ GTG TGC GGG TTA TCC TGC ATT AGC 3′ | 5′ ATC GAA GCA GTG CCG CTT CTC ACC 3′ | 45 |
| GATA-3 | 262 | 55 | 32 | 5′ TCG GCC ATT CGT ACA TGG AA 3′ | 5′ GAG AGC CGT GGT GGA TGG AC 3′ | 71 |
| HPRT | 249 | 60 | 30 | 5′ CAC AGG ACT AGA ACA CCT GC 3′ | 5′ GCT GGT GAA AAG GAC CTC T 3′ | 69 |
| cDNA . | Length . | Tm . | Cycles . | 5′ sequence . | 3′ sequence . | Reference . |
|---|---|---|---|---|---|---|
| GATA-2 | 720 | 55 | 32 | 5′ CGG AAT TCG ACA CAC CAC CCG ATA CCC ACC TAT 3′ | 5′ CGG AAT TCG CCT ACG CCA TGG CAG TCA CCA TGC T 3′ | 67 |
| c-mpl | 322 | 60 | 30 | 5′ GCA GCA CCC AGT GGG ACA TAC CAG 3′ | 5′ GCC ATA GCG GAG TTC ATG CCT CAG G 3′ | 65 |
| NF-E2 | 400 | 60 | 34 | 5′ AGA TGG AAC TGA CTT GGC AAG 3′ | 5′ TCT GCA TCA CTG TAG TTG AGG 3′ | 66 |
| GATA-1 | 375 | 60 | 32 | 5′ GGA ATT CGG GCC CCT TGT GAG GCC AGA GAG 3′ | 5′ CGG GGT ACC TCA CGC TCC AGC CAG ATT CGA CCC 3′ | 68 |
| EPO-R | 452 | 55 | 32 | 5′ GGA CAC CTA CTT GGT ATT GG 3′ | 5′ GAC GTT GTA GGC TGG AGT CC 3′ | 69 |
| C/EBPα | 458 | 55 | 32 | 5′ TTC TAC GAG GCG GAG CCG CGG 3′ | 5′ GAT CAC CAG CGG CCG CAG CGC 3′ | 70 |
| Pax-5 | 439 | 65 | 36 | 5′ CTA CAG GCT CCG TGA CGC AG 3′ | 5′ TCT CGG CCT GTG ACA ATA GG 3′ | 72 |
| Aiolos | 463 | 65 | 32 | 5′ GTG TGC GGG TTA TCC TGC ATT AGC 3′ | 5′ ATC GAA GCA GTG CCG CTT CTC ACC 3′ | 45 |
| GATA-3 | 262 | 55 | 32 | 5′ TCG GCC ATT CGT ACA TGG AA 3′ | 5′ GAG AGC CGT GGT GGA TGG AC 3′ | 71 |
| HPRT | 249 | 60 | 30 | 5′ CAC AGG ACT AGA ACA CCT GC 3′ | 5′ GCT GGT GAA AAG GAC CTC T 3′ | 69 |