Oligonucleotide heavy-chain primers
| Primers . | Location* . | Orientation . | Sequence (5′-3′) . |
|---|---|---|---|
| CH-specific | |||
| Cμ16 | μCH1 (120-115) | Antisense | TTG GGG CGG ATG CAC T |
| Cδ1 | δCH1 (173-164) | Antisense | TTC TCT GGG GCT GTG TC |
| CγFF1 | γCH1 (192-184) | Antisense | GGT CAC CAC GCT GCT GAG GGA GTA GA |
| CαFF1 | αCH1 (187-179) | Antisense | CGT GGT GTA CAG GTC CCC GGA GGC A |
| Cα4 | αCH1 (125-118) | Antisense | GCT CAG CGG GAA GAC CTT GGG GCT |
| VH(patient)-specific | |||
| 163-CDR3 | V1-02 (92-99) | Sense | GTG CGT CGC TCC CAT ATG ATT ACA |
| 163-CDR1 | V1-02 (27-35) | Sense | CAC CTT CAC CGG CTA CTA TAT GCA |
| 103-CDR3 | V4-31 (92-100) | Sense | TGT GCG AGA GAT TTT ACC CTT CCG A |
| 103-CDR1 | V4-34 (32-37) | Sense | GGT GGT TAC TAC TGG AGC TGG G |
| 83-CDR3 | V3-23 (92-99) | Sense | TGT GCG AAA GAT TGG GCT GGG GA |
| 83-CDR1 | V3-23 (26-34) | Sense | GGA TTC ACC TTT AGC AGC TAT GCC A |
| 42-CDR3 | V3-33 (90-99) | Sense | CTA CTG TGC GAG AGA TAT GGG GCT A |
| 42-CDR1 | V3-33 (24-31) | Sense | GCG TCT GGA TTC CCC TTC AGT GA |
| 93-CDR3 | V3-23 (91-99) | Sense | TAT TGT GCG AGA GAT CAA CAC AGT A |
| 93-CDR1 | V3-23 (27-34) | Sense | TTC ATC TTC AGT ACC TAT GGC ATG |
| β-actin RNA | |||
| 5′ H-βA | Exon 2 | Sense | TGA TAT CGC CGC GCT CGT |
| 3′ H-βA | Exon 4 | Antisense | CTC GGC CGT GGT GGT GAA |
| Primers . | Location* . | Orientation . | Sequence (5′-3′) . |
|---|---|---|---|
| CH-specific | |||
| Cμ16 | μCH1 (120-115) | Antisense | TTG GGG CGG ATG CAC T |
| Cδ1 | δCH1 (173-164) | Antisense | TTC TCT GGG GCT GTG TC |
| CγFF1 | γCH1 (192-184) | Antisense | GGT CAC CAC GCT GCT GAG GGA GTA GA |
| CαFF1 | αCH1 (187-179) | Antisense | CGT GGT GTA CAG GTC CCC GGA GGC A |
| Cα4 | αCH1 (125-118) | Antisense | GCT CAG CGG GAA GAC CTT GGG GCT |
| VH(patient)-specific | |||
| 163-CDR3 | V1-02 (92-99) | Sense | GTG CGT CGC TCC CAT ATG ATT ACA |
| 163-CDR1 | V1-02 (27-35) | Sense | CAC CTT CAC CGG CTA CTA TAT GCA |
| 103-CDR3 | V4-31 (92-100) | Sense | TGT GCG AGA GAT TTT ACC CTT CCG A |
| 103-CDR1 | V4-34 (32-37) | Sense | GGT GGT TAC TAC TGG AGC TGG G |
| 83-CDR3 | V3-23 (92-99) | Sense | TGT GCG AAA GAT TGG GCT GGG GA |
| 83-CDR1 | V3-23 (26-34) | Sense | GGA TTC ACC TTT AGC AGC TAT GCC A |
| 42-CDR3 | V3-33 (90-99) | Sense | CTA CTG TGC GAG AGA TAT GGG GCT A |
| 42-CDR1 | V3-33 (24-31) | Sense | GCG TCT GGA TTC CCC TTC AGT GA |
| 93-CDR3 | V3-23 (91-99) | Sense | TAT TGT GCG AGA GAT CAA CAC AGT A |
| 93-CDR1 | V3-23 (27-34) | Sense | TTC ATC TTC AGT ACC TAT GGC ATG |
| β-actin RNA | |||
| 5′ H-βA | Exon 2 | Sense | TGA TAT CGC CGC GCT CGT |
| 3′ H-βA | Exon 4 | Antisense | CTC GGC CGT GGT GGT GAA |
Parentheses represent codons aligned by the primers. Numbering is according to criteria defined by Chothia et al.61