Primers and probes used for real-time polymerase chain reaction
| Gene . | Primer 1 (5′-3′) . | Primer 2 (5′-3′) . | Probe (5′-FAM-3′-TAMRA) . |
|---|---|---|---|
| CASP8 | AAGCAAACCTCGGGGATACT | TCCATTCCTGTCCCTAATGC | AGCACGGGAGAAAGTGCCCAAA |
| CASP9 | GTTGAGACCCTGGACGACAT | CCTTTCACCGAAACAGCATT | CAGTCCCTCCTGCTTAGGGTCGC |
| RAIDD | TGGAGGGACTGGTTCTTCAG | ATCCAGCAGGAGCATTGTTT | TGCTCAAACCACAGGCCTCCG |
| DAXX | GACGGACATTTCCTCTTCCA | CGCCTCCATTGAAGGAAGTA | CAATCAGAGGAGCCCTTCACCACTG |
| DP2 | AACCATTTGGCTGCTGATTC | GATTCTGACATTCCTGAGCAGAA | CAAGTGGATTGGCCTGCCTACCA |
| FADD | GTGCGGGAGTCACTGAGAAT | GTCAGCCACCAGGTTCATCT | AACGCAACAGTGGCCCACCTG |
| FAS | TCCAAGGGATTGGAATTGAG | ACCTGGAGGACAGGGCTTAT | AGGCCTGCATCATGATGGCCA |
| ICSBP1 | GCTGATCAAGGAGCCTTCTG | ACCAGTCTGGAAGGAGCTGA | AAAAGGAGCCCTTCCCCGCC |
| PDCD1 | TGTCTTTCCTAGCGGAATGG | CCAAGAGCAGTGTCCATCCT | TCGGAGTGCCCAGCCACTGA |
| STAF50 | TCATTTGCTGGGTTTGTGAA | GCTACCTGCAGCTTTTCCTG | CCAAGGTCACCAAACATTCCGC |
| TRAIL | TCACAGTGCTCCTGCAGTCT | GCAATGCCACTTTTGGAGTA | CCAACGAGCTGAAGCAGATGCA |
| BAC | TCCCTGGAGAAGAGCTACGA | AGGAAGGAAGGCTGGAAGAG | CAATGAGCGGTTCCGCTGCC |
| Gene . | Primer 1 (5′-3′) . | Primer 2 (5′-3′) . | Probe (5′-FAM-3′-TAMRA) . |
|---|---|---|---|
| CASP8 | AAGCAAACCTCGGGGATACT | TCCATTCCTGTCCCTAATGC | AGCACGGGAGAAAGTGCCCAAA |
| CASP9 | GTTGAGACCCTGGACGACAT | CCTTTCACCGAAACAGCATT | CAGTCCCTCCTGCTTAGGGTCGC |
| RAIDD | TGGAGGGACTGGTTCTTCAG | ATCCAGCAGGAGCATTGTTT | TGCTCAAACCACAGGCCTCCG |
| DAXX | GACGGACATTTCCTCTTCCA | CGCCTCCATTGAAGGAAGTA | CAATCAGAGGAGCCCTTCACCACTG |
| DP2 | AACCATTTGGCTGCTGATTC | GATTCTGACATTCCTGAGCAGAA | CAAGTGGATTGGCCTGCCTACCA |
| FADD | GTGCGGGAGTCACTGAGAAT | GTCAGCCACCAGGTTCATCT | AACGCAACAGTGGCCCACCTG |
| FAS | TCCAAGGGATTGGAATTGAG | ACCTGGAGGACAGGGCTTAT | AGGCCTGCATCATGATGGCCA |
| ICSBP1 | GCTGATCAAGGAGCCTTCTG | ACCAGTCTGGAAGGAGCTGA | AAAAGGAGCCCTTCCCCGCC |
| PDCD1 | TGTCTTTCCTAGCGGAATGG | CCAAGAGCAGTGTCCATCCT | TCGGAGTGCCCAGCCACTGA |
| STAF50 | TCATTTGCTGGGTTTGTGAA | GCTACCTGCAGCTTTTCCTG | CCAAGGTCACCAAACATTCCGC |
| TRAIL | TCACAGTGCTCCTGCAGTCT | GCAATGCCACTTTTGGAGTA | CCAACGAGCTGAAGCAGATGCA |
| BAC | TCCCTGGAGAAGAGCTACGA | AGGAAGGAAGGCTGGAAGAG | CAATGAGCGGTTCCGCTGCC |
Primers and TaqMan probes were designed using software PRIMER3 (http://www.genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi) using sequence data from the NCBI database.