Sequences and SH3 affinity measurements with W fluorescence of peptides used in this study
| Nomenclature . | Sequence . | Kd (μM) Grb2SH3(N) . |
|---|---|---|
| P1 (hu. SoS1 aa 1152-1162) | VPPPVPPRRRP-amide | 11.63 ± 2.44 |
| P2 (HAGBP) | PPPPLPPRRRR-amide | 2.72 ± 0.18 |
| P3 (Antp) | GTERQIKIWFPNRRMKWKKEN | NA |
| P4 (Antp-HAGBP) | GPPPPLPPRRRR-GTERQIKIWFPNRRMKWKKEN-amide | NA |
| P5 (Antp-Ctrl. LL) | GPPPPLPPLLRR-GTERQIKIWFPNRRMKWKKEN-amide | NA |
| P6 (Antp-SS-HAGBP) | KKWKMRRNPFWIKIQRC-CGIRVVDNSPPPPLPPRRRRSAPSPTRV-amide | 0.78 ± 0.03* |
| P7 (Antp-SS-Ctrl. GGLL) | KKWKMRRNPFWIKIQRC-CGIRVVDNSPPGALGPLLRRSAPSPTRV-amide | NB* |
| Nomenclature . | Sequence . | Kd (μM) Grb2SH3(N) . |
|---|---|---|
| P1 (hu. SoS1 aa 1152-1162) | VPPPVPPRRRP-amide | 11.63 ± 2.44 |
| P2 (HAGBP) | PPPPLPPRRRR-amide | 2.72 ± 0.18 |
| P3 (Antp) | GTERQIKIWFPNRRMKWKKEN | NA |
| P4 (Antp-HAGBP) | GPPPPLPPRRRR-GTERQIKIWFPNRRMKWKKEN-amide | NA |
| P5 (Antp-Ctrl. LL) | GPPPPLPPLLRR-GTERQIKIWFPNRRMKWKKEN-amide | NA |
| P6 (Antp-SS-HAGBP) | KKWKMRRNPFWIKIQRC-CGIRVVDNSPPPPLPPRRRRSAPSPTRV-amide | 0.78 ± 0.03* |
| P7 (Antp-SS-Ctrl. GGLL) | KKWKMRRNPFWIKIQRC-CGIRVVDNSPPGALGPLLRRSAPSPTRV-amide | NB* |
HAGBP indicates high-affinity Grb2-binding peptide; NA, not applicable (W in shuttle); NB, no binding.
Measured without W-containing shuttle sequence.