Effect of TNF on noncycling SRCs
| . | −TNF . | +TNF . | ||
|---|---|---|---|---|
| Percent human engraftment . | Positive mice . | Percent human engraftment . | Positive mice . | |
| Exp 1 | 0.8 (0.1-1.6) | 1/2 | 3 (4.5-1.4) | 2/2 |
| Exp 2 | 0.8 (0.5-0.9-1) | 3/3 | 0.8 (0-0.6-1.8) | 2/3 |
| . | −TNF . | +TNF . | ||
|---|---|---|---|---|
| Percent human engraftment . | Positive mice . | Percent human engraftment . | Positive mice . | |
| Exp 1 | 0.8 (0.1-1.6) | 1/2 | 3 (4.5-1.4) | 2/2 |
| Exp 2 | 0.8 (0.5-0.9-1) | 3/3 | 0.8 (0-0.6-1.8) | 2/3 |
CD34+ CB cells were cultured for 5 days in X-vivo 15 supplemented with 1% BSA and Tpo 200 ng/mL in the absence or presence of TNF (20 ng/mL). Each NOD-SCID mouse was transplanted with the equivalent of 50 000 CD34+ cells. Data are presented as number of mice positive for human reconstitution with both B and myeloid cells. Also shown is the percentage human engraftment (representing total human reconstitution independent of whether both myeloid and B cells were observed) of individual animals within each group.
Exp indicates experiment.