Characters of synthetic oligopeptides and results with the MHC stabilization assay
Peptide no. . | Amino acid sequence . | ORF name . | Character . | Start position . | Amino acid length . | Score* . | % MFI increase† . |
---|---|---|---|---|---|---|---|
1 | RYTAESLRL | UL69 | IE | 259 | 9 | 400 | 227 |
2 | DYEPVPRKF | 33 | 9 | 277 | 141 | ||
3 | VYNAPNLHTL | 329 | 10 | 300 | 196 | ||
4 | AYCPFDEQSL | 517 | 10 | 288 | 92 | ||
5 | DYLLHIRQQF | 301 | 10 | 252 | 123 | ||
6 | TYRCGAVSDL | 477 | 10 | 200 | 58 | ||
7 | VYLFSLVVL | US3 | IE | 164 | 9 | 300 | 175 |
8 | VYDLANNTL | IRS1 | IE | 218 | 9 | 240 | 194 |
9 | RYDDESWRPL | 640 | 10 | 480 | 36 | ||
10 | FYQIRKPPWL | 598 | 10 | 300 | 217 | ||
11 | VYDLANNTL | TRS1 | IE | 218 | 9 | 240 | 136 |
12 | LYLALGAGF | 206 | 9 | 180 | 79 | ||
13 | RYTRPTEPPL | 631 | 10 | 400 | 17 | ||
14 | AYVCCQEYL | UL36 | Teg | 69 | 9 | 300 | 172 |
15 | LYDAITENL | 350 | 9 | 280 | 202 | ||
16 | CYHQLLGAL | 231 | 9 | 240 | 114 | ||
17 | LYRGDVESL | 156 | 9 | 200 | 155 | ||
18 | KYAESDYIF | UL37 | IE | 353 | 9 | 240 | 177 |
19 | KYAESDYIFL | 353 | 10 | 480 | 145 | ||
20 | SYRWIQRKRL | 21 | 10 | 200 | −20 | ||
21 | QYHLEGWFPL | 262 | 10 | 200 | 109 | ||
22 | QYILGADPL | UL123 | IE | 353 | 9 | 300 | 189 |
23 | KYTQTEEKF | 107 | 9 | 220 | 142 | ||
24 | AYAQKIFKIL | 248 | 10 | 336 | 152 | ||
25 | AYLGLSKKL | UL48 | Teg | 433 | 9 | 396 | 146 |
26 | DYLRFPTRL | 2026 | 9 | 300 | 104 | ||
27 | EYHGVYEHL | 563 | 9 | 280 | 144 | ||
28 | TYRDRDIDL | 223 | 9 | 240 | −21 | ||
29 | TYSTLDRAL | 470 | 9 | 240 | 165 | ||
30 | FYDLRDLKL | 662 | 9 | 220 | 121 | ||
31 | TYAQMVKKL | 880 | 9 | 220 | 212 | ||
32 | GYQKKVQQDL | 1016 | 10 | 504 | −29 | ||
33 | LYPEYIYTVL | 1753 | 10 | 504 | 161 | ||
34 | TYVLVTVNSL | 136 | 10 | 360 | 203 | ||
35 | YYEALFLYML | 197 | 10 | 300 | 215 | ||
36 | DYGNVAFKYL | 1609 | 10 | 240 | 47 | ||
37 | KYCDPNVLL | UL56 | Teg | 27 | 9 | 480 | 149 |
38 | LYLLLYRHL | 756 | 9 | 360 | 157 | ||
39 | TYNKEWPLL | 735 | 9 | 300 | 202 | ||
40 | IYELCNGPL | 617 | 9 | 300 | 133 | ||
41 | MYTKIKHIL | 397 | 9 | 280 | 155 | ||
42 | VYHQLCRAL | 320 | 9 | 240 | 148 | ||
43 | LYLEMLLKAL | 72 | 10 | 432 | 36 | ||
44 | LYNETFGKQL | 708 | 10 | 360 | 34 | ||
45 | SYIQEAEEAL | 269 | 10 | 300 | −9 | ||
46 | TYNKEWPLLL | 735 | 10 | 300 | 238 | ||
47 | AYIYTTYLL | UL55 | gB | 154 | 9 | 300 | 239 |
48 | SYENKTMQL | 205 | 9 | 300 | 116 | ||
49 | AYEYVDYLF | 624 | 9 | 210 | 225 | ||
50 | DYLFKRMIDL | 629 | 10 | 300 | −2.4 | ||
51 | IYNKPIAARF | 531 | 10 | 210 | 169 | ||
52 | SYAYIYTTYL | 152 | 10 | 200 | 228 | ||
53 | QYRSYKRSL | UL100 | gM | 174 | 9 | 200 | −16 |
54 | GYHLGAFFGL | 268 | 10 | 240 | 157 | ||
55 | YYHVVDFERL | 46 | 10 | 240 | 196 | ||
56 | KYESWLRPL | UL115 | gL | 92 | 9 | 600 | 103 |
57 | YYAGLPPEL | 277 | 9 | 264 | 196 | ||
58 | KYYAGLPPEL | 276 | 10 | 528 | 172 | ||
59 | LYNAVKEFCL | 253 | 10 | 300 | 185 | ||
60 | NYLDLSALL | UL75 | gH | 306 | 9 | 432 | 192 |
61 | SYVVTNQYL | 589 | 9 | 360 | 209 | ||
62 | SYLKDSDFL | 291 | 9 | 300 | 174 | ||
63 | TYALVSKDL | 125 | 9 | 280 | 178 | ||
64 | SYRSFSQQL | 135 | 9 | 240 | 82 | ||
65 | TYGRPIRFL | 45 | 9 | 200 | 169 | ||
66 | YYVFHMPRCL | 88 | 10 | 300 | 227 | ||
67 | MYMHDSDDVL | 668 | 10 | 300 | 133 | ||
68 | HYFTTLKQYL | UL18 | Env | 344 | 10 | 288 | 182 |
69 | EYTRLGVWL | US27 | Env | 31 | 9 | 240 | 91 |
70 | EYAKHHPKL | 89 | 9 | 220 | 33 | ||
71 | IYQLFEYTRL | 26 | 10 | 300 | 169 | ||
72 | QYLLDHNSL | US28 | Env | 91 | 9 | 360 | 161 |
73 | FYVAMFASL | 111 | 9 | 360 | 172 | ||
74 | LYVFVGTKF | 290 | 9 | 231 | 71 | ||
75 | RYRPVKQACL | 137 | 10 | 480 | 117 | ||
76 | SYPIILNVEL | 183 | 10 | 396 | 183 | ||
77 | TYNCTYNRL | UL4 | Env | 110 | 9 | 360 | 122 |
78 | HYRYEVANL | 183 | 10 | 200 | −22 | ||
79 | TYNRLTLLNL | 114 | 10 | 300 | 59 | ||
80 | VYALPLKML | UL83 | Teg | 100 | 9 | 200 | 151 |
81 | QYDPVAALF | 328 | 9 | 168 | 215 | ||
82 | QYVKVYLESF | 209 | 10 | 150 | 26 | ||
83 | QYDPVAALFF | 328 | 10 | 120 | 185 | ||
84 | RYLRDQQLL‡ | 584 | 9 | 720 | 141 | ||
85 | TYGPVFMSL1-153 | 287 | 9 | 403 | 229 |
Peptide no. . | Amino acid sequence . | ORF name . | Character . | Start position . | Amino acid length . | Score* . | % MFI increase† . |
---|---|---|---|---|---|---|---|
1 | RYTAESLRL | UL69 | IE | 259 | 9 | 400 | 227 |
2 | DYEPVPRKF | 33 | 9 | 277 | 141 | ||
3 | VYNAPNLHTL | 329 | 10 | 300 | 196 | ||
4 | AYCPFDEQSL | 517 | 10 | 288 | 92 | ||
5 | DYLLHIRQQF | 301 | 10 | 252 | 123 | ||
6 | TYRCGAVSDL | 477 | 10 | 200 | 58 | ||
7 | VYLFSLVVL | US3 | IE | 164 | 9 | 300 | 175 |
8 | VYDLANNTL | IRS1 | IE | 218 | 9 | 240 | 194 |
9 | RYDDESWRPL | 640 | 10 | 480 | 36 | ||
10 | FYQIRKPPWL | 598 | 10 | 300 | 217 | ||
11 | VYDLANNTL | TRS1 | IE | 218 | 9 | 240 | 136 |
12 | LYLALGAGF | 206 | 9 | 180 | 79 | ||
13 | RYTRPTEPPL | 631 | 10 | 400 | 17 | ||
14 | AYVCCQEYL | UL36 | Teg | 69 | 9 | 300 | 172 |
15 | LYDAITENL | 350 | 9 | 280 | 202 | ||
16 | CYHQLLGAL | 231 | 9 | 240 | 114 | ||
17 | LYRGDVESL | 156 | 9 | 200 | 155 | ||
18 | KYAESDYIF | UL37 | IE | 353 | 9 | 240 | 177 |
19 | KYAESDYIFL | 353 | 10 | 480 | 145 | ||
20 | SYRWIQRKRL | 21 | 10 | 200 | −20 | ||
21 | QYHLEGWFPL | 262 | 10 | 200 | 109 | ||
22 | QYILGADPL | UL123 | IE | 353 | 9 | 300 | 189 |
23 | KYTQTEEKF | 107 | 9 | 220 | 142 | ||
24 | AYAQKIFKIL | 248 | 10 | 336 | 152 | ||
25 | AYLGLSKKL | UL48 | Teg | 433 | 9 | 396 | 146 |
26 | DYLRFPTRL | 2026 | 9 | 300 | 104 | ||
27 | EYHGVYEHL | 563 | 9 | 280 | 144 | ||
28 | TYRDRDIDL | 223 | 9 | 240 | −21 | ||
29 | TYSTLDRAL | 470 | 9 | 240 | 165 | ||
30 | FYDLRDLKL | 662 | 9 | 220 | 121 | ||
31 | TYAQMVKKL | 880 | 9 | 220 | 212 | ||
32 | GYQKKVQQDL | 1016 | 10 | 504 | −29 | ||
33 | LYPEYIYTVL | 1753 | 10 | 504 | 161 | ||
34 | TYVLVTVNSL | 136 | 10 | 360 | 203 | ||
35 | YYEALFLYML | 197 | 10 | 300 | 215 | ||
36 | DYGNVAFKYL | 1609 | 10 | 240 | 47 | ||
37 | KYCDPNVLL | UL56 | Teg | 27 | 9 | 480 | 149 |
38 | LYLLLYRHL | 756 | 9 | 360 | 157 | ||
39 | TYNKEWPLL | 735 | 9 | 300 | 202 | ||
40 | IYELCNGPL | 617 | 9 | 300 | 133 | ||
41 | MYTKIKHIL | 397 | 9 | 280 | 155 | ||
42 | VYHQLCRAL | 320 | 9 | 240 | 148 | ||
43 | LYLEMLLKAL | 72 | 10 | 432 | 36 | ||
44 | LYNETFGKQL | 708 | 10 | 360 | 34 | ||
45 | SYIQEAEEAL | 269 | 10 | 300 | −9 | ||
46 | TYNKEWPLLL | 735 | 10 | 300 | 238 | ||
47 | AYIYTTYLL | UL55 | gB | 154 | 9 | 300 | 239 |
48 | SYENKTMQL | 205 | 9 | 300 | 116 | ||
49 | AYEYVDYLF | 624 | 9 | 210 | 225 | ||
50 | DYLFKRMIDL | 629 | 10 | 300 | −2.4 | ||
51 | IYNKPIAARF | 531 | 10 | 210 | 169 | ||
52 | SYAYIYTTYL | 152 | 10 | 200 | 228 | ||
53 | QYRSYKRSL | UL100 | gM | 174 | 9 | 200 | −16 |
54 | GYHLGAFFGL | 268 | 10 | 240 | 157 | ||
55 | YYHVVDFERL | 46 | 10 | 240 | 196 | ||
56 | KYESWLRPL | UL115 | gL | 92 | 9 | 600 | 103 |
57 | YYAGLPPEL | 277 | 9 | 264 | 196 | ||
58 | KYYAGLPPEL | 276 | 10 | 528 | 172 | ||
59 | LYNAVKEFCL | 253 | 10 | 300 | 185 | ||
60 | NYLDLSALL | UL75 | gH | 306 | 9 | 432 | 192 |
61 | SYVVTNQYL | 589 | 9 | 360 | 209 | ||
62 | SYLKDSDFL | 291 | 9 | 300 | 174 | ||
63 | TYALVSKDL | 125 | 9 | 280 | 178 | ||
64 | SYRSFSQQL | 135 | 9 | 240 | 82 | ||
65 | TYGRPIRFL | 45 | 9 | 200 | 169 | ||
66 | YYVFHMPRCL | 88 | 10 | 300 | 227 | ||
67 | MYMHDSDDVL | 668 | 10 | 300 | 133 | ||
68 | HYFTTLKQYL | UL18 | Env | 344 | 10 | 288 | 182 |
69 | EYTRLGVWL | US27 | Env | 31 | 9 | 240 | 91 |
70 | EYAKHHPKL | 89 | 9 | 220 | 33 | ||
71 | IYQLFEYTRL | 26 | 10 | 300 | 169 | ||
72 | QYLLDHNSL | US28 | Env | 91 | 9 | 360 | 161 |
73 | FYVAMFASL | 111 | 9 | 360 | 172 | ||
74 | LYVFVGTKF | 290 | 9 | 231 | 71 | ||
75 | RYRPVKQACL | 137 | 10 | 480 | 117 | ||
76 | SYPIILNVEL | 183 | 10 | 396 | 183 | ||
77 | TYNCTYNRL | UL4 | Env | 110 | 9 | 360 | 122 |
78 | HYRYEVANL | 183 | 10 | 200 | −22 | ||
79 | TYNRLTLLNL | 114 | 10 | 300 | 59 | ||
80 | VYALPLKML | UL83 | Teg | 100 | 9 | 200 | 151 |
81 | QYDPVAALF | 328 | 9 | 168 | 215 | ||
82 | QYVKVYLESF | 209 | 10 | 150 | 26 | ||
83 | QYDPVAALFF | 328 | 10 | 120 | 185 | ||
84 | RYLRDQQLL‡ | 584 | 9 | 720 | 141 | ||
85 | TYGPVFMSL1-153 | 287 | 9 | 403 | 229 |
ORF indicates open reading frame; IE, immediate early genes; Teg, (possible) tegument proteins; g, glycoproteins; Env, (possible) envelope proteins.
Estimated half-time of dissociation from the HLA-A24 allele (min).
Percent MFI increase of HLA-A*2402 molecules on T2-A24 cells. Percent MFI increase = (MFI with sample peptide − MFI without peptide)/MFI without peptide) × 100. See the MHC stabilization assay in “Materials and methods.”
Derived from the HIV envelope protein.31
Derived from the EBV latent membrane protein 2A.32