CEACAM1 primers for site-directed mutagenesis
| Name . | Sequence 5′…3′ . |
|---|---|
| BGPAMPs | CAUCAUCAUCAUAAGCTTATGGGGCACCTC |
| NTMas | GCCATTTTCTTGGGGCAGCTCCGGGTATAC |
| NTMs | GTATACCCGGAGCTGCCCCAAGAAAATGGC |
| Trans-cyt-as | CUACUACUACUAAGACTATGAAGTTGGTTG |
| MutS32s | CTTTTTGGCTACGCCTGGTACAAAGG |
| MutS32as | CCTTTGTACCAGGCGTAGCCAAAAAG |
| MutY34s | TGGCTACAGCTGGGCCAAAGGGGAAAG |
| MutY34as | CTTTCCCCTTTGGCCAGCTGTAGCCA |
| MutV39s | GGAAAGAGCGGATGGCAA |
| MutV39as | TTGCCATCCGCTCTTTCC |
| MutQ44s | GGATGGCAACCGTGCAATTGTAGGATATGC |
| MutQ44as | GCATATCCTACAATTGCACGGTTGCCATCC |
| MutT87s | GGATTCTACGCCCTACAAGTC |
| MutT87as | GACTTGTAGGGCGTAGAATCC |
| MutQ89s | GGATTCTACACCCTAGCAGTCATAAAGTCAG |
| MutQ89as | CTGACTTTATGACTGCTAGGGCGTAGAATCC |
| MutI91s | CACCCTACAAGTCGCAAAGTCAGATCTTG |
| MutI91as | CAAGATCTGACTTTGCGACTTGTAGGGTG |
| MutV96s | GATCTTGTGAATGCAGAAGCAACTG |
| MutV96as | CAGTTGCTTCTGCATTCACAAGATC |
| MutV96E98s | GTCAGATCTTGCGAATGCAGAAGCAACTG |
| MutV96E98as | CAGTTGCTTCTGCATTCGCAAGATCTGAC |
| MutS32Y34V39s | GCAACTTTTTGGCTACGCCTGGGCCAAAGGGGAAAGAGCG |
| MutS32Y34V39as | CGCTCTTTCCCCTTTGGCCCAGGCGTAGCCAAAAAGTTGC |
| MutT87Q89I91s | CACAGGATTCTACGCCCTAGCAGTCGCAAAGTCAG |
| MutT87Q89I91as | CTGACTTTGCGACTGCTAGGGCGTAGAATCCTGTG |
| MutL18L20s | GAGGGGAAGGAGGTTGCTCTCGCTGTCCACAATCTGCCCC |
| MutL18L20as | GGGGCAGATTGTGGACAGCGAGAGCAACCTCCTTCCCCTC |
| MutS72L74s | CAATATACCCCAATGCAGCCCTGGCGATCCAGAACGTCACCC |
| MutS72L74as | GGGTGACGTTCTGGATCGCCAGGGCTGCATTGGGGTATATTG |
| IIH3Ms | GAGAACCCACTGCTTAACTGG |
| BGPNA1B1as | CTGATCCGGAGAATTCCTTACCTGTAGTGACTATGATCGTCTTGACTGT |
| MutR38As | GGTACAAAGGGGAAGCAGTGGATGGCAACCG |
| MutR38Aas | CGGTTGCCATCCACTGCTTCCCCTTTGTACC |
| MutD40As | GGGGAAAGAGTGGCCGGCAACCGTCAA |
| MutD40Aas | TTGACGGTTGCCGGCCACTCTTTCCCC |
| MutR64Ds | CCGCAAACAGCGGTGACGAGACAATATACCC |
| MutR64Das | GGGTATATTGTCTC GTCACCGCTGTTTGCGG |
| MutD82As | GAACGTCACCCAGAATGCCACAGGATTCTACAC |
| MutD82Aas | GTGTAGAATCCTGTGGCATTCTGGGTGACGTTC |
| MutD82Rs | GAACGTCACCCAGAATCGCACAGGATTCTACAC |
| MutD82Ras | GTGTAGAATCCTGTGCGATTCTGGGTGACGTTC |
| Name . | Sequence 5′…3′ . |
|---|---|
| BGPAMPs | CAUCAUCAUCAUAAGCTTATGGGGCACCTC |
| NTMas | GCCATTTTCTTGGGGCAGCTCCGGGTATAC |
| NTMs | GTATACCCGGAGCTGCCCCAAGAAAATGGC |
| Trans-cyt-as | CUACUACUACUAAGACTATGAAGTTGGTTG |
| MutS32s | CTTTTTGGCTACGCCTGGTACAAAGG |
| MutS32as | CCTTTGTACCAGGCGTAGCCAAAAAG |
| MutY34s | TGGCTACAGCTGGGCCAAAGGGGAAAG |
| MutY34as | CTTTCCCCTTTGGCCAGCTGTAGCCA |
| MutV39s | GGAAAGAGCGGATGGCAA |
| MutV39as | TTGCCATCCGCTCTTTCC |
| MutQ44s | GGATGGCAACCGTGCAATTGTAGGATATGC |
| MutQ44as | GCATATCCTACAATTGCACGGTTGCCATCC |
| MutT87s | GGATTCTACGCCCTACAAGTC |
| MutT87as | GACTTGTAGGGCGTAGAATCC |
| MutQ89s | GGATTCTACACCCTAGCAGTCATAAAGTCAG |
| MutQ89as | CTGACTTTATGACTGCTAGGGCGTAGAATCC |
| MutI91s | CACCCTACAAGTCGCAAAGTCAGATCTTG |
| MutI91as | CAAGATCTGACTTTGCGACTTGTAGGGTG |
| MutV96s | GATCTTGTGAATGCAGAAGCAACTG |
| MutV96as | CAGTTGCTTCTGCATTCACAAGATC |
| MutV96E98s | GTCAGATCTTGCGAATGCAGAAGCAACTG |
| MutV96E98as | CAGTTGCTTCTGCATTCGCAAGATCTGAC |
| MutS32Y34V39s | GCAACTTTTTGGCTACGCCTGGGCCAAAGGGGAAAGAGCG |
| MutS32Y34V39as | CGCTCTTTCCCCTTTGGCCCAGGCGTAGCCAAAAAGTTGC |
| MutT87Q89I91s | CACAGGATTCTACGCCCTAGCAGTCGCAAAGTCAG |
| MutT87Q89I91as | CTGACTTTGCGACTGCTAGGGCGTAGAATCCTGTG |
| MutL18L20s | GAGGGGAAGGAGGTTGCTCTCGCTGTCCACAATCTGCCCC |
| MutL18L20as | GGGGCAGATTGTGGACAGCGAGAGCAACCTCCTTCCCCTC |
| MutS72L74s | CAATATACCCCAATGCAGCCCTGGCGATCCAGAACGTCACCC |
| MutS72L74as | GGGTGACGTTCTGGATCGCCAGGGCTGCATTGGGGTATATTG |
| IIH3Ms | GAGAACCCACTGCTTAACTGG |
| BGPNA1B1as | CTGATCCGGAGAATTCCTTACCTGTAGTGACTATGATCGTCTTGACTGT |
| MutR38As | GGTACAAAGGGGAAGCAGTGGATGGCAACCG |
| MutR38Aas | CGGTTGCCATCCACTGCTTCCCCTTTGTACC |
| MutD40As | GGGGAAAGAGTGGCCGGCAACCGTCAA |
| MutD40Aas | TTGACGGTTGCCGGCCACTCTTTCCCC |
| MutR64Ds | CCGCAAACAGCGGTGACGAGACAATATACCC |
| MutR64Das | GGGTATATTGTCTC GTCACCGCTGTTTGCGG |
| MutD82As | GAACGTCACCCAGAATGCCACAGGATTCTACAC |
| MutD82Aas | GTGTAGAATCCTGTGGCATTCTGGGTGACGTTC |
| MutD82Rs | GAACGTCACCCAGAATCGCACAGGATTCTACAC |
| MutD82Ras | GTGTAGAATCCTGTGCGATTCTGGGTGACGTTC |
s indicates forward primer; as, reverse primer.