Gene-specific primers for RT-PCR
| Gene . | Orientation . | Sequence . |
|---|---|---|
| VEGF | Forward | 5′-CGA AGT GGT GAA GTT CAT GGA TG-3′ |
| Reverse | 5′-TTC TGT ATC AGT CTT TCC TGG TGA G-3′ | |
| VEGFR-1 | Forward | 5′-CAA GTG GCC AGA GGC ATG GAG TT-3′ |
| Reverse | 5′-GAT GTA GTC TTT ACC ATC CTG TTG-3′ | |
| VEGFR-2 | Forward | 5′-GAG GGC CTC TCA TGG TGA TTG T-3′ |
| Reverse | 5′-TGC CAG CAG TCC AGC ATG GTC TG-3′ | |
| β-actin | Forward | 5′-GTG GGG CGC CCC AGG CAC CA-3′ |
| Reverse | 5′-CTC CTT AAT GTC ACG CAC GAT TTC-3′ |
| Gene . | Orientation . | Sequence . |
|---|---|---|
| VEGF | Forward | 5′-CGA AGT GGT GAA GTT CAT GGA TG-3′ |
| Reverse | 5′-TTC TGT ATC AGT CTT TCC TGG TGA G-3′ | |
| VEGFR-1 | Forward | 5′-CAA GTG GCC AGA GGC ATG GAG TT-3′ |
| Reverse | 5′-GAT GTA GTC TTT ACC ATC CTG TTG-3′ | |
| VEGFR-2 | Forward | 5′-GAG GGC CTC TCA TGG TGA TTG T-3′ |
| Reverse | 5′-TGC CAG CAG TCC AGC ATG GTC TG-3′ | |
| β-actin | Forward | 5′-GTG GGG CGC CCC AGG CAC CA-3′ |
| Reverse | 5′-CTC CTT AAT GTC ACG CAC GAT TTC-3′ |