Morphology of fetal liver cell lines under different culture conditions
| Cell types and conditions . | Blasts . | Promyelocytes . | Immature neutrophils . | Segmented neutrophils . |
|---|---|---|---|---|
| FL-1(+/−) SCF, IL-3 | 68 | 27 | 3 | 2 |
| FL-1(+/−) GM-CSF | 18 | 32 | 21 | 29 |
| FL-1(+/−) SCF, IL-3 + ATRA | 53 | 39 | 6 | 2 |
| FL-8(−/−) SCF, IL-3 | 43 | 33 | 13 | 11 |
| FL-8(−/−) GM-CSF | 15 | 22 | 25 | 38 |
| FL-8(−/−) SCF, IL-3 + ATRA | 29 | 20 | 30 | 21 |
| Cell types and conditions . | Blasts . | Promyelocytes . | Immature neutrophils . | Segmented neutrophils . |
|---|---|---|---|---|
| FL-1(+/−) SCF, IL-3 | 68 | 27 | 3 | 2 |
| FL-1(+/−) GM-CSF | 18 | 32 | 21 | 29 |
| FL-1(+/−) SCF, IL-3 + ATRA | 53 | 39 | 6 | 2 |
| FL-8(−/−) SCF, IL-3 | 43 | 33 | 13 | 11 |
| FL-8(−/−) GM-CSF | 15 | 22 | 25 | 38 |
| FL-8(−/−) SCF, IL-3 + ATRA | 29 | 20 | 30 | 21 |
Cytospins of the fetal liver cell lines cultured with the indicated growth factors were stained with Wright-Giemsa. Numbers are the percentages of cells of the indicated morphology with a minimum of 300 cells assessed for each culture. For GM-CSF induction, cells continuously growing in SCF and IL-3 were washed, resuspended in GM-CSF, and cultured for an additional 4 days. ATRA induction (10 μM) was for 5 days.
Immature neutrophils include myelocytes, metamyelocytes, and bands (“doughnut” nuclei).