Primers and probes used for polymerase chain reaction amplification of TCRD rearrangements and real-time quantitative polymerase chain reaction
| Primer . | Sequence (5′-3′) . | Reference . |
|---|---|---|
| Coding joint PCR | ||
| Vδ1-5′⁗ | ACT CAA GCC CAG TCA TCA GTA TCC | 33 |
| Vδ2-5′ | ACC AAA CAG TGC CTG TGT CAA TAG G | 33 |
| Vδ3-5′ | GAC CAG ACG GTG GCG AGT GGC | 33 |
| Dδ2-5′ RO2 | CCA CAT TGG GAG TGT CAA CAT TT | This study |
| Dδ3-3′ N | CTT CCT GCT ATC CCT TCC AGG | 33 |
| Dδ3-5′ | CGC GTC GAC CAT ATA GTG GAA ACC GAG GGG | This study |
| Jδ1-3′ | ACC TCT TCC CAG GAG TCC TCC | 33 |
| Jδ3-3′ | CGC GTC GAC TCA AAT TAT CCC AGA AAT ATA GG | 44 |
| δREC-3′ | GCA ACA TCA CTC TGT GTC TAG C | 35 |
| ψJα-3′ | CCT GAA GCT TAA GGC ACA TTA GAA TCT CTC ACT G | 35 |
| Signal joint PCR | ||
| Vδ2-sj 3′ | CTG GTC AGT GGT TTT TGA GCT GCT | This study |
| Dδ2-5′ XBg | GTA GAT CTA GAA GAG GGT TTT TAT ACT GAT GTG | This study |
| Dδ2-lower | TCC CAA TGC TGA GAC ATA CAT | This study |
| Dδ3-upper | CAG GGG CCA TAT AGT TGT GAA | This study |
| Dδ3-5′ S | CGC GTC GA CCA TAT AGT GTG AAA CCG AGG GG | This study |
| Dδ3-3′ N | CTT CCT GCT ATC CCT TCC AGG | 33 |
| Jδ1 sj 5′ | GTC CCT ACC TGC AGA TGA TTA ACC | This study |
| Jδ3 sj 5′ | CCC TTG GTC TCA TCA AGA GCA GC | This study |
| Real-time PCR | ||
| F-DD2-KLON | GAA GAA GAG GGT TTT TAT ACT GAT GTG TT | This study |
| R-DD3-CONS4 | TTG CCC CTG CAG TTT TTG TAC | This study |
| T-DD3-CONS2 | ATA CGC ACA GTG CTA CAA AAC CTA CAG AGA CCT | This study |
| F-VG8-KLON | TCT ATT ACT GTG CCA CCT GGG ATA | This study |
| R-JG13/23-KLON | TTC CTG CCT TCC CTC TAT TAC CTT | This study |
| T-JG13/23-CONS3 | TGT CAC AGG TAA GTA TCG GAA GAA TAC AAC ATT TCC | This study |
| Primer . | Sequence (5′-3′) . | Reference . |
|---|---|---|
| Coding joint PCR | ||
| Vδ1-5′⁗ | ACT CAA GCC CAG TCA TCA GTA TCC | 33 |
| Vδ2-5′ | ACC AAA CAG TGC CTG TGT CAA TAG G | 33 |
| Vδ3-5′ | GAC CAG ACG GTG GCG AGT GGC | 33 |
| Dδ2-5′ RO2 | CCA CAT TGG GAG TGT CAA CAT TT | This study |
| Dδ3-3′ N | CTT CCT GCT ATC CCT TCC AGG | 33 |
| Dδ3-5′ | CGC GTC GAC CAT ATA GTG GAA ACC GAG GGG | This study |
| Jδ1-3′ | ACC TCT TCC CAG GAG TCC TCC | 33 |
| Jδ3-3′ | CGC GTC GAC TCA AAT TAT CCC AGA AAT ATA GG | 44 |
| δREC-3′ | GCA ACA TCA CTC TGT GTC TAG C | 35 |
| ψJα-3′ | CCT GAA GCT TAA GGC ACA TTA GAA TCT CTC ACT G | 35 |
| Signal joint PCR | ||
| Vδ2-sj 3′ | CTG GTC AGT GGT TTT TGA GCT GCT | This study |
| Dδ2-5′ XBg | GTA GAT CTA GAA GAG GGT TTT TAT ACT GAT GTG | This study |
| Dδ2-lower | TCC CAA TGC TGA GAC ATA CAT | This study |
| Dδ3-upper | CAG GGG CCA TAT AGT TGT GAA | This study |
| Dδ3-5′ S | CGC GTC GA CCA TAT AGT GTG AAA CCG AGG GG | This study |
| Dδ3-3′ N | CTT CCT GCT ATC CCT TCC AGG | 33 |
| Jδ1 sj 5′ | GTC CCT ACC TGC AGA TGA TTA ACC | This study |
| Jδ3 sj 5′ | CCC TTG GTC TCA TCA AGA GCA GC | This study |
| Real-time PCR | ||
| F-DD2-KLON | GAA GAA GAG GGT TTT TAT ACT GAT GTG TT | This study |
| R-DD3-CONS4 | TTG CCC CTG CAG TTT TTG TAC | This study |
| T-DD3-CONS2 | ATA CGC ACA GTG CTA CAA AAC CTA CAG AGA CCT | This study |
| F-VG8-KLON | TCT ATT ACT GTG CCA CCT GGG ATA | This study |
| R-JG13/23-KLON | TTC CTG CCT TCC CTC TAT TAC CTT | This study |
| T-JG13/23-CONS3 | TGT CAC AGG TAA GTA TCG GAA GAA TAC AAC ATT TCC | This study |
PCR indicates polymerase chain reaction; sj, signal joint.