Frequency of NSCs from human fetal brain in response to EGF, FGF-2, and EGF plus FGF-2
| Donor no. . | EGF . | FGF-2 . | EGF + FGF-2 . | |||
|---|---|---|---|---|---|---|
| Primary cultures . | Secondary cultures . | Primary cultures . | Secondary cultures . | Primary cultures . | Secondary cultures . | |
| 1 | 21.8 ± 5.3 | 90.3 ± 7.3 | 17.7 ± 6.1 | 72.4 ± 5.4 | 32.8 ± 5.2 | 132.4 ± 9.2 |
| 2 | 22.2 ± 6.1 | 90.4 ± 6.6 | 16.8 ± 5.8 | 70.6 ± 6.3 | 33.1 ± 6.2 | 133.8 ± 9.8 |
| 3 | 23.2 ± 5.4 | 92.8 ± 6.4 | 17.6 ± 4.2 | 72.6 ± 6.4 | 31.8 ± 5.6 | 130.5 ± 8.6 |
| Donor no. . | EGF . | FGF-2 . | EGF + FGF-2 . | |||
|---|---|---|---|---|---|---|
| Primary cultures . | Secondary cultures . | Primary cultures . | Secondary cultures . | Primary cultures . | Secondary cultures . | |
| 1 | 21.8 ± 5.3 | 90.3 ± 7.3 | 17.7 ± 6.1 | 72.4 ± 5.4 | 32.8 ± 5.2 | 132.4 ± 9.2 |
| 2 | 22.2 ± 6.1 | 90.4 ± 6.6 | 16.8 ± 5.8 | 70.6 ± 6.3 | 33.1 ± 6.2 | 133.8 ± 9.8 |
| 3 | 23.2 ± 5.4 | 92.8 ± 6.4 | 17.6 ± 4.2 | 72.6 ± 6.4 | 31.8 ± 5.6 | 130.5 ± 8.6 |
Cells were plated in 96-well plates, either as 5000 cells/200 μL per well in the primary cultures or as 500 cells/200 μL per well in the secondary cultures. These wells were scored 7 days later for the presence of spheres. The total number of wells analyzed is 300 for each primary culture and 200 for each secondary culture. Data for the number of spheres per well are presented as the mean ± SD in each culture condition. These data are compiled from 3 independent experiments using different donor samples. EGF indicates epidermal growth factor; FGF-2, fibroblast growth factor.