Cellular composition of 6 clonally derived EGF-generated neurospheres which contain cells for all 3 lineages including neurons, astrocytes, and oligodendrocytes
| Clonally derived spheres . | Percentage of lineage-specific cells in the sphere . | ||
|---|---|---|---|
| MAP-2–positive . | GFAP-positive . | O4-positive . | |
| 1 | 8.2% (26/316) | 56.3% (178/316) | 2.5% (8/316) |
| 2 | 7.8% (31/396) | 59.3% (235/396) | 3.0% (12/396) |
| 3 | 8.4% (36/429) | 60.6% (260/429) | 3.3% (14/429) |
| 4 | 8.1% (28/347) | 58.8% (204/347) | 2.9% (10/347) |
| 5 | 7.2% (25/346) | 62.4% (216/346) | 2.9% (10/346) |
| 6 | 7.2% (30/417) | 64.3% (268/417) | 2.9% (12/417) |
| Mean ± SEM | 7.8 ± 0.5% | 60.3 ± 2.8% | 2.9 ± 0.2% |
| Clonally derived spheres . | Percentage of lineage-specific cells in the sphere . | ||
|---|---|---|---|
| MAP-2–positive . | GFAP-positive . | O4-positive . | |
| 1 | 8.2% (26/316) | 56.3% (178/316) | 2.5% (8/316) |
| 2 | 7.8% (31/396) | 59.3% (235/396) | 3.0% (12/396) |
| 3 | 8.4% (36/429) | 60.6% (260/429) | 3.3% (14/429) |
| 4 | 8.1% (28/347) | 58.8% (204/347) | 2.9% (10/347) |
| 5 | 7.2% (25/346) | 62.4% (216/346) | 2.9% (10/346) |
| 6 | 7.2% (30/417) | 64.3% (268/417) | 2.9% (12/417) |
| Mean ± SEM | 7.8 ± 0.5% | 60.3 ± 2.8% | 2.9 ± 0.2% |
The number of neurons (MAP-2–positive), astrocytes (GFAP-positive), and oligodendrocytes (O4-positive) was determined in 10 nonoverlapping fields in each sphere. Numbers in parentheses indicate the total number of lineage-specific cells divided by the total number of cells in each sphere. The mean ± SD represent the mean frequency for each of the 3 different lineages of cells among 6 clonally derived spheres examined. MAP-2 indicates microtubule-associated protein-2; GFAP, glial fibrillary acidic protein.