Cellular composition of different lineage brain cells among clonally derived neurospheres generated under different conditions
| Donor no. . | EGF-generated neurospheres . | FGF-2–generated neurospheres . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Primary neurospheres . | Secondary neurospheres . | Primary neurospheres . | Secondary neurospheres . | |||||||||
| MAP-2 . | GFAP . | O4 . | MAP-2 . | GFAP . | O4 . | MAP-2 . | GFAP . | O4 . | MAP-2 . | GFAP . | O4 . | |
| 1 | 7.8 ± 0.5 | 60.3 ± 2.8 | 2.9 ± 0.2 | 8.0 ± 0.6 | 60.3 ± 2.6 | 3.0 ± 0.3 | 7.6 ± 0.3 | 60.6 ± 3.1 | 2.8 ± 0.4 | 8.1 ± 0.4 | 61.8 ± 3.1 | 2.8 ± 0.4 |
| 2 | 8.1 ± 0.7 | 60.6 ± 2.2 | 3.0 ± 0.4 | 7.9 ± 0.5 | 59.8 ± 2.4 | 3.1 ± 0.5 | 7.8 ± 0.4 | 59.2 ± 2.9 | 2.8 ± 0.3 | 8.0 ± 0.2 | 58.9 ± 2.8 | 3.0 ± 0.3 |
| 3 | 7.8 ± 0.4 | 61.8 ± 2.1 | 3.0 ± 0.3 | 8.0 ± 0.6 | 59.6 ± 2.8 | 3.1 ± 0.4 | 7.7 ± 0.4 | 60.6 ± 2.8 | 2.9 ± 0.4 | 8.1 ± 0.4 | 59.9 ± 2.4 | 2.9 ± 0.4 |
| Donor no. . | EGF-generated neurospheres . | FGF-2–generated neurospheres . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Primary neurospheres . | Secondary neurospheres . | Primary neurospheres . | Secondary neurospheres . | |||||||||
| MAP-2 . | GFAP . | O4 . | MAP-2 . | GFAP . | O4 . | MAP-2 . | GFAP . | O4 . | MAP-2 . | GFAP . | O4 . | |
| 1 | 7.8 ± 0.5 | 60.3 ± 2.8 | 2.9 ± 0.2 | 8.0 ± 0.6 | 60.3 ± 2.6 | 3.0 ± 0.3 | 7.6 ± 0.3 | 60.6 ± 3.1 | 2.8 ± 0.4 | 8.1 ± 0.4 | 61.8 ± 3.1 | 2.8 ± 0.4 |
| 2 | 8.1 ± 0.7 | 60.6 ± 2.2 | 3.0 ± 0.4 | 7.9 ± 0.5 | 59.8 ± 2.4 | 3.1 ± 0.5 | 7.8 ± 0.4 | 59.2 ± 2.9 | 2.8 ± 0.3 | 8.0 ± 0.2 | 58.9 ± 2.8 | 3.0 ± 0.3 |
| 3 | 7.8 ± 0.4 | 61.8 ± 2.1 | 3.0 ± 0.3 | 8.0 ± 0.6 | 59.6 ± 2.8 | 3.1 ± 0.4 | 7.7 ± 0.4 | 60.6 ± 2.8 | 2.9 ± 0.4 | 8.1 ± 0.4 | 59.9 ± 2.4 | 2.9 ± 0.4 |
The data presented are the mean ± SD of the mean frequency for each of the 3 different lineages of cells among a total of 6 clonally derived spheres from each culture condition. The data are compiled from 3 independent experiments using different donor brain samples. For abbreviations, see Table 3 footnote.