One million neurosphere cells were injected directly into each human bone fragment in SCID mice, and BM cells were harvested from each bone fragment 4 months later. One million harvested BM cells were then injected into each human graft in the SCID-hu mice, including bone and thy/liv mice. Hematopoietic reconstitution derived from human fetal neurosphere cells was determined by flow cytometry in these SCID-hu mice 4 months later. The frequency of hematopoietic reconstitution is presented as the total number of hematopoietic-reconstituted animals divided by the total number of animals used in the experiments. The percentage of neurosphere-derived hematopoietic cells represents the mean ± SD of all hematopoietic-reconstituted animals from the same experiments. These data are compiled from 3 independent experiments using different donor brain samples.