Membrane-targeted Mpl and cytoplasmic Mpl require JAK2 for signaling in fetal liver cells
| Vector . | Colonies/105 cells . | ||
|---|---|---|---|
| Culture conditions . | JAK2 +/+ . | JAK2 −/− . | |
| None* | IL-3 | 198 ± 12 | 0 ± 0 |
| JAK2† | IL-3 plus EPO | 92, 105 | 20, 31 |
| Mpl (membrane)‡ | AP20187 | 9, 11 | 0, 0 |
| Mpl (cytoplasm)‡ | AP20187 | 8, 9 | 0, 0 |
| Vector . | Colonies/105 cells . | ||
|---|---|---|---|
| Culture conditions . | JAK2 +/+ . | JAK2 −/− . | |
| None* | IL-3 | 198 ± 12 | 0 ± 0 |
| JAK2† | IL-3 plus EPO | 92, 105 | 20, 31 |
| Mpl (membrane)‡ | AP20187 | 9, 11 | 0, 0 |
| Mpl (cytoplasm)‡ | AP20187 | 8, 9 | 0, 0 |
Results shown are the mean ± SD of the number of colonies per 105 fetal liver cells plated.
Fetal liver cells from either wild-type or JAK2-deficient day 12.5 embryos were cultured overnight with a JAK2 expressing retrovirus, then placed in colony assays. Results indicate the number of colonies per 105 cells plated from 2 animals.
Fetal liver cells from either wild-type or JAK2-deficient day 12.5 embryos were cultured overnight with a retrovirus encoding either membrane-targeted Mpl or cytoplasmic Mpl, then placed in colony assays. Results indicate the number of colonies per 105cells plated from 2 animals.