TRAIL-induced apoptosis in uninfected and HHV-7–infected SupT1 cultures
| . | Cells scored . | Apoptosis, no. . | Apoptosis, % . |
|---|---|---|---|
| Uninfected SupT1 cultures | 475 | 151 | 31.7 |
| HHV-7–infected SupT1 cultures | |||
| HHV-7 antigen–positive cells | 518 | 23 | 4.4 |
| HHV-7 antigen–negative cells | 507 | 149 | 29.3 |
| . | Cells scored . | Apoptosis, no. . | Apoptosis, % . |
|---|---|---|---|
| Uninfected SupT1 cultures | 475 | 151 | 31.7 |
| HHV-7–infected SupT1 cultures | |||
| HHV-7 antigen–positive cells | 518 | 23 | 4.4 |
| HHV-7 antigen–negative cells | 507 | 149 | 29.3 |
After treatment with recombinant TRAIL, cells were scored by fluorescence microscopy to detect apoptotic nuclei and HHV-7 antigen expression (in HHV-7–infected cultures) by using dual-label staining for cell nuclei (DAPI) and HHV-7 antigens, as described in “Materials and methods” (see also legend to Figure 7B). Data represent the results of cells scored from 4 independent experiments.