Phagocytosis of IgG-coated SRBCs by transfected CHO cells
| Clone No. . | FcγRIIA wt . | FcγRIIA tailless . | FcγRIIA mutant . | CR3 . | No. . | CN . | SRBC bound . | SRBC internal . | Fused lysosomes . |
|---|---|---|---|---|---|---|---|---|---|
| Receptors expressed | |||||||||
| 161 -24 | − | − | − | − | 3 | 352 | 4 | 0 | 0 |
| 161 -84 | − | − | − | + | 3 | 349 | 2 | 0 | 0 |
| 135 -12 | − | + | − | − | 3 | 348 | 395 | 0 | 0 |
| 131 -3* | + | − | − | − | 3 | 123 | 238 | 167 | 162 |
| 169 -23* | − | + | − | + | 5 | 584 | 672 | 54 | 4 |
| 169 -8 | − | + | − | + | 5 | 592 | 635 | 35 | 2 |
| 173 -46 | + | − | − | + | 3 | 230 | 440 | 210 | 204 |
| Receptors expressed transiently | |||||||||
| +† | − | − | + | 2 | 193 | 253 | 171 | 163 | |
| − | +‡ | − | + | 2 | 248 | 293 | 38 | 3 | |
| − | − | +1-153 | − | 4 | 936 | 693 | 41-155 | 01-155 | |
| − | − | +1-154 | + | 3 | 649 | 734 | 127 | 114 | |
| Clone No. . | FcγRIIA wt . | FcγRIIA tailless . | FcγRIIA mutant . | CR3 . | No. . | CN . | SRBC bound . | SRBC internal . | Fused lysosomes . |
|---|---|---|---|---|---|---|---|---|---|
| Receptors expressed | |||||||||
| 161 -24 | − | − | − | − | 3 | 352 | 4 | 0 | 0 |
| 161 -84 | − | − | − | + | 3 | 349 | 2 | 0 | 0 |
| 135 -12 | − | + | − | − | 3 | 348 | 395 | 0 | 0 |
| 131 -3* | + | − | − | − | 3 | 123 | 238 | 167 | 162 |
| 169 -23* | − | + | − | + | 5 | 584 | 672 | 54 | 4 |
| 169 -8 | − | + | − | + | 5 | 592 | 635 | 35 | 2 |
| 173 -46 | + | − | − | + | 3 | 230 | 440 | 210 | 204 |
| Receptors expressed transiently | |||||||||
| +† | − | − | + | 2 | 193 | 253 | 171 | 163 | |
| − | +‡ | − | + | 2 | 248 | 293 | 38 | 3 | |
| − | − | +1-153 | − | 4 | 936 | 693 | 41-155 | 01-155 | |
| − | − | +1-154 | + | 3 | 649 | 734 | 127 | 114 | |
No. indicates number of experiments performed; CN, total number of transfected CHO cells counted; SRBC bound, total number of sheep erythrocytes bound; SRBC internal, total number of internalized erythrocytes; Fused lysosomes, number of internalized SRBCs colocalized with dextran.
Data presented by combining experiments with fluorescent dextran and acid phosphatase.
Mean fluorescence intensity (MFI) of 89 for FcγRIIA wild-type transfected into CR3 permanent line.
MFI of 96 for FcγRIIA tailless transfected into CR3 permanent line.
MFI of 93 for FcγRIIA (ITAM mutant) transfected into CHO cells.
Too few internal targets to quantitate phagolysosome fusion.
MFI of 91 for FcγRIIA mutant transfected into CR3 permanent line.