Cytogenetically abnormal cells in MDS marrow before and after exposure to TRAIL as determined by FISH analysis
| Patient ID . | Diagnosis (FAB) . | Cytogenetic abnormality . | Cytogenetically abnormal cells (%) . | |
|---|---|---|---|---|
| Before TRAIL . | After TRAIL . | |||
| 255 | RA | 5q− | 84 | 0 |
| 167 | RA | +21 | 14 | 0 |
| 221 | RA | 5q− | 38 | 0 |
| 187 | RA | −20 | 51 | 29 |
| 256 | RA | −7 | 28 | 15 |
| 209 | RAEB | 5q− | 73 | 53 |
| 236 | RAEB | −7 | 9 | 4 |
| 183 | RAEB-T | +8 | 3 | 5 |
| 218 | tAML | +8 | 13 | 32 |
| Patient ID . | Diagnosis (FAB) . | Cytogenetic abnormality . | Cytogenetically abnormal cells (%) . | |
|---|---|---|---|---|
| Before TRAIL . | After TRAIL . | |||
| 255 | RA | 5q− | 84 | 0 |
| 167 | RA | +21 | 14 | 0 |
| 221 | RA | 5q− | 38 | 0 |
| 187 | RA | −20 | 51 | 29 |
| 256 | RA | −7 | 28 | 15 |
| 209 | RAEB | 5q− | 73 | 53 |
| 236 | RAEB | −7 | 9 | 4 |
| 183 | RAEB-T | +8 | 3 | 5 |
| 218 | tAML | +8 | 13 | 32 |
Fresh marrow cells from patients with known clonal cytogenetic abnormalities were cultured for 6 to 12 hours in the presence of TRAIL (300 ng/mL). After washing, FISH analysis with the appropriate probes was carried out on pre-exposure and postexposure samples, side by side.