Oligonucleotide primers used for gene expression analysis by RT-PCR
| Gene . | Size (bp) . | Annealing temperature (°C) . | 5′ primer . | 3′ primer . |
|---|---|---|---|---|
| SCL | 744 | 55 | ATTGCACACACGGGATTCTG | CATACAGTACGACACTGACG |
| GATA-1 | 581 | 55 | ATGCCTGTAATCCCAGCACT | TCATGGTGGTAGCTGGTAGC |
| GATA-2 | 720 | 60 | CGGAATTCGACACACCACCCGATACCCACCTAT | CGGAATTCGCCTACGCCATGGCAGTCACCATGCT |
| EKLF | 359 | 55 | TCGCCGGAGACGCAGGCT | CCCAGTCCTTGTGCAGGA |
| NF-E2(p45) | 391 | 62.5 | GAGCCCTGGCCATGAAGATTCC | CACCATCAGCAGCCTGTTGCAG |
| MafK(p18) | 491 | 55 | GACAGGGCCCGGGTTATGACGACTAATCCC | GGATCCGGAGGCGGCTGAGAAGGGTACAGAGGT |
| c-myb | 681 | 55 | TTCAAGGCCAGCATTCTTGC | CCTCTAGGAGCTCATTTGTG |
| AML1b | 292 | 55 | CCAGCAAGCTGAGGAGCGGCG | CCGACAAACCTGAGGTCGTTG |
| C/EBPα | 191 | 58 | AAGGCCAAGAAGTCGGTGGA | CAGTTCACGGCTCAGCTGTT |
| PU.1 | 615 | 55 | TGGAAGGGTTTTCCCTCACC | TGCTGTCCTTCATGTCGCCG |
| β-globin | 578 | 55 | CTGACAGATGCTCTCTTGGG | CACAACCCCAGAAACAGACA |
| MPO | 379 | 55 | GGTGCTGAAGAACCTGGAGTTG | CTAGGTCTCCTTCCAGGAAGTC |
| HPRT | 249 | 50 | CACAGGACTAGAACACCTGC | GCTGGTGAAAAGGACCTCT |
| Gene . | Size (bp) . | Annealing temperature (°C) . | 5′ primer . | 3′ primer . |
|---|---|---|---|---|
| SCL | 744 | 55 | ATTGCACACACGGGATTCTG | CATACAGTACGACACTGACG |
| GATA-1 | 581 | 55 | ATGCCTGTAATCCCAGCACT | TCATGGTGGTAGCTGGTAGC |
| GATA-2 | 720 | 60 | CGGAATTCGACACACCACCCGATACCCACCTAT | CGGAATTCGCCTACGCCATGGCAGTCACCATGCT |
| EKLF | 359 | 55 | TCGCCGGAGACGCAGGCT | CCCAGTCCTTGTGCAGGA |
| NF-E2(p45) | 391 | 62.5 | GAGCCCTGGCCATGAAGATTCC | CACCATCAGCAGCCTGTTGCAG |
| MafK(p18) | 491 | 55 | GACAGGGCCCGGGTTATGACGACTAATCCC | GGATCCGGAGGCGGCTGAGAAGGGTACAGAGGT |
| c-myb | 681 | 55 | TTCAAGGCCAGCATTCTTGC | CCTCTAGGAGCTCATTTGTG |
| AML1b | 292 | 55 | CCAGCAAGCTGAGGAGCGGCG | CCGACAAACCTGAGGTCGTTG |
| C/EBPα | 191 | 58 | AAGGCCAAGAAGTCGGTGGA | CAGTTCACGGCTCAGCTGTT |
| PU.1 | 615 | 55 | TGGAAGGGTTTTCCCTCACC | TGCTGTCCTTCATGTCGCCG |
| β-globin | 578 | 55 | CTGACAGATGCTCTCTTGGG | CACAACCCCAGAAACAGACA |
| MPO | 379 | 55 | GGTGCTGAAGAACCTGGAGTTG | CTAGGTCTCCTTCCAGGAAGTC |
| HPRT | 249 | 50 | CACAGGACTAGAACACCTGC | GCTGGTGAAAAGGACCTCT |