Table 1. Gene expression analysis by... | American Society of Hematology

Table 1.

Gene expression analysis by real-time quantitative RT-PCR (genes increased in FL)

Gene	HUGO	C_TGC ± SEM	C_TFL ± SEM	ΔC_T	P
Bsp-1^*	MADH1	29.2 ± 0.7	22.8 ± 1.6	6.4	.0001
Bcl-2^*	BCL2	26.1 ± 0.9	20.2 ± 1.4	5.9	.0001
Mlk-3^*	MLK3	36.1 ± 2.9	31.5 ± 2.5	4.6	.04
p21CIP1^*	CDKN1A	26.5 ± 0.7	23.4 ± 1.2	3.1	.002
Hsp27	HSPB1	28.1 ± 1.1	25.4 ± 1.7	2.7	.04
Hsp40	HSPF1	24.8 ± 0.2	22.2 ± 2.1	2.6	.04
TNF	TNF	21.8 ± 0.8	19.3 ± 1.5	2.5	.02
Id-2^*	ID2	27.3 ± 1.0	24.8 ± 1.3	2.4	.02
c-Jun	JUN	24.5 ± 0.9	22.2 ± 1.9	2.3	.05
Ear-2^*	ERBAL2	26.5 ± 0.4	24.4 ± 1.1	2.1	.005
YY1	YY1	28.4 ± 0.8	26.3 ± 1.2	2.1	.02
CDK10^*	CDK10	26.3 ± 0.6	24.7 ± 0.7	1.6	.01
ZFX	ZFX	28.6 ± 0.6	27.1 ± 0.9	1.5	.03
IL-4R-α	IL4R	18.1 ± 0.2	16.6 ± 0.4	1.5	.0004
Db1	ZNF161	25.2 ± 1.0	23.8 ± 0.7	1.3	.09
IL-2R-γ^*	IL2RG	22.5 ± 0.7	21.2 ± 0.5	1.3	.05
Xpb^*	ERCC3	24.0 ± 0.3	22.9 ± 0.7	1.1	.02
CCN1B^*	CCN1B	28.6 ± 0.8	26.5 ± 1.3	1.1	.03
Pax-5	PAX5	23.0 ± 0.3	21.7 ± 1.0	1.1	.08
Lyn^*	LYN	21.5 ± 0.5	20.5 ± 0.6	1.0	.08
SNF2-α^*	SMARCA2	23.3 ± 0.2	22.3 ± 1.0	1.0	.08
p16INK4A^*	CDKN2A	22.1 ± 0.1	21.1 ± 0.8	0.9	.09
p120^*	NOL1	21.4 ± 0.5	20.7 ± 0.5	0.7	.08
Hsf-1^*	HSF1	19.7 ± 0.2	19.1 ± 0.1	0.6	.004

Gene	HUGO	C_TGC ± SEM	C_TFL ± SEM	ΔC_T	P
Bsp-1^*	MADH1	29.2 ± 0.7	22.8 ± 1.6	6.4	.0001
Bcl-2^*	BCL2	26.1 ± 0.9	20.2 ± 1.4	5.9	.0001
Mlk-3^*	MLK3	36.1 ± 2.9	31.5 ± 2.5	4.6	.04
p21CIP1^*	CDKN1A	26.5 ± 0.7	23.4 ± 1.2	3.1	.002
Hsp27	HSPB1	28.1 ± 1.1	25.4 ± 1.7	2.7	.04
Hsp40	HSPF1	24.8 ± 0.2	22.2 ± 2.1	2.6	.04
TNF	TNF	21.8 ± 0.8	19.3 ± 1.5	2.5	.02
Id-2^*	ID2	27.3 ± 1.0	24.8 ± 1.3	2.4	.02
c-Jun	JUN	24.5 ± 0.9	22.2 ± 1.9	2.3	.05
Ear-2^*	ERBAL2	26.5 ± 0.4	24.4 ± 1.1	2.1	.005
YY1	YY1	28.4 ± 0.8	26.3 ± 1.2	2.1	.02
CDK10^*	CDK10	26.3 ± 0.6	24.7 ± 0.7	1.6	.01
ZFX	ZFX	28.6 ± 0.6	27.1 ± 0.9	1.5	.03
IL-4R-α	IL4R	18.1 ± 0.2	16.6 ± 0.4	1.5	.0004
Db1	ZNF161	25.2 ± 1.0	23.8 ± 0.7	1.3	.09
IL-2R-γ^*	IL2RG	22.5 ± 0.7	21.2 ± 0.5	1.3	.05
Xpb^*	ERCC3	24.0 ± 0.3	22.9 ± 0.7	1.1	.02
CCN1B^*	CCN1B	28.6 ± 0.8	26.5 ± 1.3	1.1	.03
Pax-5	PAX5	23.0 ± 0.3	21.7 ± 1.0	1.1	.08
Lyn^*	LYN	21.5 ± 0.5	20.5 ± 0.6	1.0	.08
SNF2-α^*	SMARCA2	23.3 ± 0.2	22.3 ± 1.0	1.0	.08
p16INK4A^*	CDKN2A	22.1 ± 0.1	21.1 ± 0.8	0.9	.09
p120^*	NOL1	21.4 ± 0.5	20.7 ± 0.5	0.7	.08
Hsf-1^*	HSF1	19.7 ± 0.2	19.1 ± 0.1	0.6	.004

For each of the 6 individuals and the 6 patient samples, a real-time quantitative RT-PCR was performed for all the genes presented in Figure 2. The mean values are shown for GC (C_TGC) ± SEM and FL (C_TFL) ± SEM as well as the difference between the means (ΔC_T). Note that a lower C_Tvalue is indicative of a higher mRNA content. A paired ttest was performed and only the genes for which the P value is less than .1 are presented here.

Confirmed in validation sample.

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