Gene expression analysis by real-time quantitative RT-PCR (genes decreased in FL)
| Gene . | HUGO . | CTGC ± SEM . | CTFL ± SEM . | ΔCT . | P . |
|---|---|---|---|---|---|
| MRP14* | S100A9 | 22.1 ± 2.1 | 30.1 ± 2.2 | −7.9 | .0006 |
| MRP8* | S100A8 | 22.5 ± 2.5 | 22.5 ± 2.9 | −6.6 | .005 |
| TDPX2* | PAGA | 22.6 ± 0.7 | 22.6 ± 0.5 | −1.7 | .01 |
| p55CDC* | CDC20 | 24.4 ± 0.6 | 24.4 ± 1.1 | −1.6 | .05 |
| CD40 | TNFRSF5 | 23.0 ± 0.5 | 23.7 ± 0.3 | −0.7 | .07 |
| Thymosin β-10* | TMSB10 | 14.9 ± 0.5 | 15.5 ± 0.4 | −0.7 | .08 |
| DBI | DBI | 18.3 ± 0.4 | 19.0 ± 0.4 | −0.7 | .03 |
| LFA-1 | ITGAL | 25.3 ± 0.8 | 25.8 ± 0.8 | −0.5 | .06 |
| Gene . | HUGO . | CTGC ± SEM . | CTFL ± SEM . | ΔCT . | P . |
|---|---|---|---|---|---|
| MRP14* | S100A9 | 22.1 ± 2.1 | 30.1 ± 2.2 | −7.9 | .0006 |
| MRP8* | S100A8 | 22.5 ± 2.5 | 22.5 ± 2.9 | −6.6 | .005 |
| TDPX2* | PAGA | 22.6 ± 0.7 | 22.6 ± 0.5 | −1.7 | .01 |
| p55CDC* | CDC20 | 24.4 ± 0.6 | 24.4 ± 1.1 | −1.6 | .05 |
| CD40 | TNFRSF5 | 23.0 ± 0.5 | 23.7 ± 0.3 | −0.7 | .07 |
| Thymosin β-10* | TMSB10 | 14.9 ± 0.5 | 15.5 ± 0.4 | −0.7 | .08 |
| DBI | DBI | 18.3 ± 0.4 | 19.0 ± 0.4 | −0.7 | .03 |
| LFA-1 | ITGAL | 25.3 ± 0.8 | 25.8 ± 0.8 | −0.5 | .06 |
Real-time quantitative RT-PCR was performed as described in Figure 2 for all the genes presented in Figure 1. The mean CT values are shown for (CTGC) ± SEM and FL (CTFL) ± SEM as well as the difference between the means (ΔCT). Note that a higher CT is indicative of a lower mRNA content. A paired t test was performed and only the genes for which the P value is less than .1 are presented here.
Confirmed in validation sample.