Binding of anti-CD44 mAbs A3D8, H90, and J173 to human myeloid cell lines
| Anti-CD44 mAb . | Relative MFI of CD44 (relative values, arbitrary units) . | |||
|---|---|---|---|---|
| KG1a . | THP1 . | HL60 . | NB4 . | |
| A3D8 | 501 ± 26 | 190 ± 12 | 246 ± 19 | 295 ± 16 |
| H90 | 553 ± 58 | 178 ± 32 | 274 ± 9 | 311 ± 18 |
| J173 | 495 ± 20 | 195 ± 28 | 277 ± 15 | 282 ± 45 |
| Anti-CD44 mAb . | Relative MFI of CD44 (relative values, arbitrary units) . | |||
|---|---|---|---|---|
| KG1a . | THP1 . | HL60 . | NB4 . | |
| A3D8 | 501 ± 26 | 190 ± 12 | 246 ± 19 | 295 ± 16 |
| H90 | 553 ± 58 | 178 ± 32 | 274 ± 9 | 311 ± 18 |
| J173 | 495 ± 20 | 195 ± 28 | 277 ± 15 | 282 ± 45 |
KG1a, THP-1, HL60, and NB4 cells are incubated with anti-CD44 FITC-conjugated A3D8, H90, or J173 mAbs. The MFI of CD44 is measured by flow cytometry as described in “Material and methods.” Data are the mean of MFI ± 1 SD calculated from 3 independent experiments.