Enhancement of LPS-induced monocytic response by LBP depletion of severe sepsis sera
| . | LBP concentration, mg/L* . | LPS binding, FU† . | TNF-α secretion, pg/mL‡ . |
|---|---|---|---|
| Severe sepsis sera | |||
| Native | 60.5 ± 38.2 | 36.7 ± 17.9 | 544 ± 228 |
| Partially LBP-depleted | 16.7 ± 10.1 | 65.1 ± 28.02-153 | 991 ± 2452-153 |
| . | LBP concentration, mg/L* . | LPS binding, FU† . | TNF-α secretion, pg/mL‡ . |
|---|---|---|---|
| Severe sepsis sera | |||
| Native | 60.5 ± 38.2 | 36.7 ± 17.9 | 544 ± 228 |
| Partially LBP-depleted | 16.7 ± 10.1 | 65.1 ± 28.02-153 | 991 ± 2452-153 |
Data are presented as mean ± SD of 8 independent experiments with randomly chosen sera of severe sepsis patients.
LBP serum concentrations were assessed by ELISA.
Mean fluorescence units were obtained by FACS analysis of gated monocytes incubated with FITC-LPS in the presence of 15% native or partially LBP-depleted severe sepsis sera.
Monocytes were stimulated with 10 ng/mL LPS in the presence of 15% native or partially LBP-depleted severe sepsis sera, and TNF-α concentrations in the supernatants were assessed by ELISA.
The statistical significance of the difference between groups was assessed by the Mann-Whitney U test;P < .05.