Primer sequences used in PCR amplifications
| Primer . | Sequence . |
|---|---|
| Vectorette | |
| 224 | CGAATCGTAACCGTTCGTACGAGAATCGCT |
| Factor VIII intron 1 primers | |
| 1F | GGGAGCTAAAGATATTTTAGAGAA |
| 1R | AAATAAATCCAATAGCCAACAAG |
| 2F | TTGTCATATGGCATGAGAGAGGGAT |
| 2R | CAATTCATTTGATAAAGCCAAC |
| 3F | GCAGCTGGTATCATCTGAATTG |
| 3R | ATGCACCATTGCTGGTAGAGG |
| 4F | AGGGCTGCACTCCTTAGTGA |
| 4R | GAACTTGAGGTGGGCAAAGATTTC |
| 5F | GAAATCTTTGCCCACCTCAA |
| 5R | GGTTTTCCCATGCTGTTCAC |
| 6F | AATCATGGTGGAAGGCAAAG |
| 6R | GGAACACTCATACTTTGGTAGTG |
| 7F | TTGGATTGTTTCCCCTTTTG |
| 7R | CCAACATGAAGAAACCCTGTCTAC |
| 8F | GCTCTTGATCCTGTTCCCTCTCTG |
| 8R | GATCTGAGGCGGACAGTTTC |
| 9F | GTTGTTGGGAATGGTTACGG |
| 9R | CACCACATCCAAACTCATGC |
| 10F | GAGGCAAGTTGTCTGGACCTATATAG |
| 10R | CCATCCTGGCTAACATGGTGAAACCC |
| 11F | TTTGTTTGTTCGTTTGCATCT |
| 11R | GCATTTGTCTTTTCATGACTGG |
| 12F | CATGCTACGACATAGATGAACC |
| 12R | TGGCAGCTGCACTTTTTAACTGC |
| Factor VIII intron 1 segment 9 primers | |
| 9aF | TTCCTAACAGGCCACAGACC |
| 9aR | CACCCGAGAAAGCACGTAGT |
| 9bF | GGAGTGAAATGCAGGGCTAC |
| 9bR | TCAGGTTGCAGACAAAGCAG |
| 9cF | GCGTTTTCCCTCAGTTACCA |
| 9cR | CTAGCTTGAGCTCCCTGTGG |
| 9dF | GAGGCAAGTTGTCTGGACCT |
| 9dR | ATGGCTTACAAAGGGCATGA |
| Int1h-2 primers | |
| Int1h-2F | GGCAGGGATCTTGTTGGTAAA |
| Int1h-2R | TGGGTGATATAAGCTGCTGAGCTA |
| Int1h repeat primers | |
| Int1hF | GCCCTCAGCCAGTACAGAAAGTCA |
| Int1hR | CCTTTGGGAGAATTCATTGCCAGCT |
| Factor VIII-VBP1 chimeric mRNA primers | |
| FVIII 1A | TGGGAGCTAAAGATATTTTAGAGAA |
| VBP1 PRIMEX1 | ACTTATACTTCTGGTACTGTTCATCCAGC |
| FVIII 1C (nested) | GAATTAACCTTTTGCTTCTCCAGTTGAAC |
| VBP1 3-M (nested) | TGTTTCATGAAGGAATCTAC |
| Primer . | Sequence . |
|---|---|
| Vectorette | |
| 224 | CGAATCGTAACCGTTCGTACGAGAATCGCT |
| Factor VIII intron 1 primers | |
| 1F | GGGAGCTAAAGATATTTTAGAGAA |
| 1R | AAATAAATCCAATAGCCAACAAG |
| 2F | TTGTCATATGGCATGAGAGAGGGAT |
| 2R | CAATTCATTTGATAAAGCCAAC |
| 3F | GCAGCTGGTATCATCTGAATTG |
| 3R | ATGCACCATTGCTGGTAGAGG |
| 4F | AGGGCTGCACTCCTTAGTGA |
| 4R | GAACTTGAGGTGGGCAAAGATTTC |
| 5F | GAAATCTTTGCCCACCTCAA |
| 5R | GGTTTTCCCATGCTGTTCAC |
| 6F | AATCATGGTGGAAGGCAAAG |
| 6R | GGAACACTCATACTTTGGTAGTG |
| 7F | TTGGATTGTTTCCCCTTTTG |
| 7R | CCAACATGAAGAAACCCTGTCTAC |
| 8F | GCTCTTGATCCTGTTCCCTCTCTG |
| 8R | GATCTGAGGCGGACAGTTTC |
| 9F | GTTGTTGGGAATGGTTACGG |
| 9R | CACCACATCCAAACTCATGC |
| 10F | GAGGCAAGTTGTCTGGACCTATATAG |
| 10R | CCATCCTGGCTAACATGGTGAAACCC |
| 11F | TTTGTTTGTTCGTTTGCATCT |
| 11R | GCATTTGTCTTTTCATGACTGG |
| 12F | CATGCTACGACATAGATGAACC |
| 12R | TGGCAGCTGCACTTTTTAACTGC |
| Factor VIII intron 1 segment 9 primers | |
| 9aF | TTCCTAACAGGCCACAGACC |
| 9aR | CACCCGAGAAAGCACGTAGT |
| 9bF | GGAGTGAAATGCAGGGCTAC |
| 9bR | TCAGGTTGCAGACAAAGCAG |
| 9cF | GCGTTTTCCCTCAGTTACCA |
| 9cR | CTAGCTTGAGCTCCCTGTGG |
| 9dF | GAGGCAAGTTGTCTGGACCT |
| 9dR | ATGGCTTACAAAGGGCATGA |
| Int1h-2 primers | |
| Int1h-2F | GGCAGGGATCTTGTTGGTAAA |
| Int1h-2R | TGGGTGATATAAGCTGCTGAGCTA |
| Int1h repeat primers | |
| Int1hF | GCCCTCAGCCAGTACAGAAAGTCA |
| Int1hR | CCTTTGGGAGAATTCATTGCCAGCT |
| Factor VIII-VBP1 chimeric mRNA primers | |
| FVIII 1A | TGGGAGCTAAAGATATTTTAGAGAA |
| VBP1 PRIMEX1 | ACTTATACTTCTGGTACTGTTCATCCAGC |
| FVIII 1C (nested) | GAATTAACCTTTTGCTTCTCCAGTTGAAC |
| VBP1 3-M (nested) | TGTTTCATGAAGGAATCTAC |