SDF-1 chemokine is not required for migration of T-cell precursors into the thymus
| Treatment* . | Presence of rhSDF-1α or α-CXCR4 . | ||
|---|---|---|---|
| Hanging drop . | FTOC . | No. of human cells per lobe after 18 d of FTOC† . | |
| Control | No | No | 26 830 |
| Control IgG2a | No | Yes | 25 690 |
| Control IgG2a | Yes | No | 30 520 |
| rhSDF-1α | No | Yes | 98 315 |
| rhSDF-1α | Yes | No | 26 963 |
| rhSDF-1α | Yes | Yes | 115 312 |
| Antihuman CXCR4 | No | Yes | 6 213 |
| Antihuman CXCR4 | Yes | No | 22 210 |
| Antihuman CXCR4 | Yes | Yes | 6 004 |
| Treatment* . | Presence of rhSDF-1α or α-CXCR4 . | ||
|---|---|---|---|
| Hanging drop . | FTOC . | No. of human cells per lobe after 18 d of FTOC† . | |
| Control | No | No | 26 830 |
| Control IgG2a | No | Yes | 25 690 |
| Control IgG2a | Yes | No | 30 520 |
| rhSDF-1α | No | Yes | 98 315 |
| rhSDF-1α | Yes | No | 26 963 |
| rhSDF-1α | Yes | Yes | 115 312 |
| Antihuman CXCR4 | No | Yes | 6 213 |
| Antihuman CXCR4 | Yes | No | 22 210 |
| Antihuman CXCR4 | Yes | Yes | 6 004 |
Comparative analysis of the influence of rhSDF-1 or antihuman CXCR4 treatment on the development of human cells in chimeric human-mouse FTOC as function of time of addition of the recombinant human protein or of the blocking mAb.
Data represent the mean human cell number per lobe from 2 to 4 independent experiments.