Table 1. Percentages of human (h) CD45-... | American Society of Hematology

Table 1.

Percentages of human (h) CD45- and GFP-expressing cells in NOD/SCID mice after engraftment of lentiviral-transduced human CB CD34⁺ cells

Mouse ID no.	Weeks	Bone marrow		Spleen
Mouse ID no.	Weeks	hCD45⁺	GFP⁺ in hCD45⁺	hCD45⁺	GFP⁺ in hCD45⁺
Mock-transduced			(Background)		(Background)
12	6	8.4	1.0^*	2.6	0.3
14	6	0.8	1.0	0.8	2.6
11	10	18.3	0.2^*	1.7	0.8^*
13	10	4.4	0.8	0.7	1.0
Mean ± SE	(n = 4)	8.0 ± 3.3	0.8 ± 0.2	1.5 ± 0.4	1.2 ± 0.4
DR.GFP-transduced
10	6	4.0	43.1^*	1.4	0.6
16	6	11.2	62.1^*	3.3	16.6
17	6	10.8	70.0	1.4	55.8^*
19	6	1.8	11.8	0.7	4.6
15	10	20.8	77.7^*	2.1	66.1^*
18	10	7.2	70.5^*	2.6	49.0
Mean ± SE	(n = 6)	9.3 ± 2.5	55.9 ± 9.2	1.9 ± 0.4	32.1 ± 10.5
EF.GFP-transduced
21	6	4.7	33.3^*	1.8	41.6^*
23	6	3.4	17.0	1.3	4.3
24	6	0.7	10.5	0.7	4.7
20	10	18.9	88.0^*	1.8	56.6^*
22	10	13.8	41.0^*	0.7	50.1
Mean ± SE	(n = 5)	8.3 ± 3.1	38.0 ± 12.2	1.3 ± 0.2	31.5 ± 10.1

Mouse ID no.	Weeks	Bone marrow		Spleen
Mouse ID no.	Weeks	hCD45⁺	GFP⁺ in hCD45⁺	hCD45⁺	GFP⁺ in hCD45⁺
Mock-transduced			(Background)		(Background)
12	6	8.4	1.0^*	2.6	0.3
14	6	0.8	1.0	0.8	2.6
11	10	18.3	0.2^*	1.7	0.8^*
13	10	4.4	0.8	0.7	1.0
Mean ± SE	(n = 4)	8.0 ± 3.3	0.8 ± 0.2	1.5 ± 0.4	1.2 ± 0.4
DR.GFP-transduced
10	6	4.0	43.1^*	1.4	0.6
16	6	11.2	62.1^*	3.3	16.6
17	6	10.8	70.0	1.4	55.8^*
19	6	1.8	11.8	0.7	4.6
15	10	20.8	77.7^*	2.1	66.1^*
18	10	7.2	70.5^*	2.6	49.0
Mean ± SE	(n = 6)	9.3 ± 2.5	55.9 ± 9.2	1.9 ± 0.4	32.1 ± 10.5
EF.GFP-transduced
21	6	4.7	33.3^*	1.8	41.6^*
23	6	3.4	17.0	1.3	4.3
24	6	0.7	10.5	0.7	4.7
20	10	18.9	88.0^*	1.8	56.6^*
22	10	13.8	41.0^*	0.7	50.1
Mean ± SE	(n = 5)	8.3 ± 3.1	38.0 ± 12.2	1.3 ± 0.2	31.5 ± 10.1

Percentages of GFP⁺ in hCD45⁺cells were determined from human cell-enriched samples via murine CD45 and Ter119 depletion.