Cytokine production in supernatants of HTLV-2/CMo-treated TF-1 cultures
Cytokine . | TF-1 + IL-3 . | TF-1 − IL-3 . | TF-1 − IL-3 + CMo . |
---|---|---|---|
IL-2 | Neg | Neg | Neg |
IL-3 | 1300 | 40 | Neg |
IL-5 | Neg | Neg | Neg |
IL-6 | Neg | Neg | Neg |
IL-10 | Neg | Neg | Neg |
IL-15 | Neg | Neg | Neg |
TNF-α | Neg | Neg | Neg |
IFN-α | Neg | 10 | Neg |
IFN-γ | Neg | 2 | 19 |
GM-CSF | Neg | Neg | 1040 |
SCF | Neg | Neg | 77 |
GH | Neg | Neg | Neg |
PRL | Neg | Neg | Neg |
Cytokine . | TF-1 + IL-3 . | TF-1 − IL-3 . | TF-1 − IL-3 + CMo . |
---|---|---|---|
IL-2 | Neg | Neg | Neg |
IL-3 | 1300 | 40 | Neg |
IL-5 | Neg | Neg | Neg |
IL-6 | Neg | Neg | Neg |
IL-10 | Neg | Neg | Neg |
IL-15 | Neg | Neg | Neg |
TNF-α | Neg | Neg | Neg |
IFN-α | Neg | 10 | Neg |
IFN-γ | Neg | 2 | 19 |
GM-CSF | Neg | Neg | 1040 |
SCF | Neg | Neg | 77 |
GH | Neg | Neg | Neg |
PRL | Neg | Neg | Neg |
Values are expressed in pM (pg/mL). Cytokine secretion was determined by ELISA in supernatants of TF-1 cells grown in IL-3, deprived of IL-3, or incubated after 24 hours of IL-3 starvation with HTLV-2 CMo for 24 hours.
TNF, tumor necrosis factor; GH, growth hormone; PRL, prolactin.