EAhy_926an is an immortalized platelet endothelial cell adhesion molecule–expressing transformed cell line; REN/PECAMs are human mesothelioma cells transfected with complementary DNA encoding human platelet endothelial cell adhesion molecules; CTM is a monoclonal antibody against human thrombomodulin.

HUVECs indicate human umbilical vein endothelial cells; PECAMs, platelet endothelial cell adhesion molecules; TM indicates thrombomodulin.

The cells were incubated with ¹²⁵I-labeled biotinylated antibodies (“no streptavidin”) or with the streptavidin-conjugated counterparts (“plus streptavidin”) for 90 minutes at 37°C. After washing, fractions of cell-surface associated and internalized iodine-125 were determined in glycin eluates and cell lysates. The data are shown as percentage of internalization, mean ± SD; n = 3.