Properties of anti–platelet endothelial cell adhesion molecule/beads
| Uncoated bead size (nm) . | Antibody-coated bead size (nm) . | Antibody/bead (no. molecules) . | Beads added per well (× 109) . | Antibody equivalent per well (× 109) . |
|---|---|---|---|---|
| 59 ± 3 | 128 ± 6 | 109 ± 8 | 300 | 3.3 ± 0.2 |
| 100 ± 3 | 150 ± 8 | 314 ± 13 | 100 | 3.1 ± 0.1 |
| 209 ± 10 | 309 ± 16 | 1372 ± 93 | 20 | 2.7 ± 0.2 |
| 477 ± 5 | 559 ± 23 | 7148 ± 106 | 12 | 8.6 ± 0.1 |
| Uncoated bead size (nm) . | Antibody-coated bead size (nm) . | Antibody/bead (no. molecules) . | Beads added per well (× 109) . | Antibody equivalent per well (× 109) . |
|---|---|---|---|---|
| 59 ± 3 | 128 ± 6 | 109 ± 8 | 300 | 3.3 ± 0.2 |
| 100 ± 3 | 150 ± 8 | 314 ± 13 | 100 | 3.1 ± 0.1 |
| 209 ± 10 | 309 ± 16 | 1372 ± 93 | 20 | 2.7 ± 0.2 |
| 477 ± 5 | 559 ± 23 | 7148 ± 106 | 12 | 8.6 ± 0.1 |
Specifications for bead size, concentration, and protein-binding capacity are from the manufacturer (Polysciences, Warrington, PA). The IgG-binding capacity of polystyrene latex beads was calculated as previously described.43 Different amounts of immunobeads were added to the cells in order to attain engagement of a similar number of platelet endothelial cell adhesion molecules per cell. This parameter is calculated as the immunoglobulin-G equivalent per well.