5-LO product formation in freshly isolated PMNLs challenged by various stimuli in presence of exogenous AA
| Stimulus . | 5-LO products (ng/106 cells) . |
|---|---|
| None | 4.2 ± 0.7 |
| Ionophore A23187 (2.5 μM) | 114.8 ± 15* |
| Thapsigargin (1 μM) | 31.2 ± 2.1* |
| FMLP (1 μM) | 18.7 ± 1.3* |
| PAF (1 μM) | 11.6 ± 4.1† |
| SA (10 μM) | 42.8 ± 2.0* |
| NaCl (0.3 M) | 50.3 ± 7.4* |
| Heat shock (45°C/20 min) | 31.5 ± 7.8* |
| Stimulus . | 5-LO products (ng/106 cells) . |
|---|---|
| None | 4.2 ± 0.7 |
| Ionophore A23187 (2.5 μM) | 114.8 ± 15* |
| Thapsigargin (1 μM) | 31.2 ± 2.1* |
| FMLP (1 μM) | 18.7 ± 1.3* |
| PAF (1 μM) | 11.6 ± 4.1† |
| SA (10 μM) | 42.8 ± 2.0* |
| NaCl (0.3 M) | 50.3 ± 7.4* |
| Heat shock (45°C/20 min) | 31.5 ± 7.8* |
The PMNLs (5 × 106 in 1 mL PGC buffer) were preincubated at 45°C for 20 minutes, with SA and NaCl at 37°C for 3 min prior to 40 μM AA, or stimulated with ionophore, thapsigargin, PAF, and fMLP together with 40 μM AA. The samples were incubated at 37°C for another 5 minutes and 5-LO products were determined by HPLC. Results are given as mean + SE (n = 4).
P < .01, Student t test.
P < .05, Student t test.