Table 4. SDF-1 triggers G 0 Inc − CD34... | American Society of Hematology

Table 4.

SDF-1 triggers G₀Inc⁻CD34⁺ cells into G₁ and makes them progress through S + G₂/M phases in synergy with Tpo and SF

Cytokines	% of PB Inc⁻CD34⁺ cells in cell cycle phases^4-150 (mean ± SD)
Cytokines	G₀	G₁	S + G₂/M
0 h culture time	28.3 ± 5.8	69.4 ± 6.2	1.9 ± 0.9
48 h culture time
SDF-1⁻
No cytokine	21.5 ± 5.1	72.2 ± 3.1	6.8 ± 1.4
SF	25.3 ± 5.1	52.4 ± 2.5	20.1 ± 3.4^‡
Tpo	20.8 ± 4.2	67.4 ± 2.8	13.1 ± 3.2^4-151
Flt-3 ligand	28.8 ± 4.8	63.8 ± 4.8	8.2 ± 3.5
SDF-1⁺
No cytokine	6.4 ± 1.8^‡	81.8 ± 4.1	11.2 ± 2.1
SF	10.5 ± 3.2^4-151	66.3 ± 2.9	22.3 ± 2.4^4-153
Tpo	8.4 ± 2.1^‡	63.8 ± 3.1	24.1 ± 3.9^4-153
Flt-3 ligand	9.1 ± 2.8	79.7 ± 3.5	12.4 ± 2.8

Cytokines	% of PB Inc⁻CD34⁺ cells in cell cycle phases^4-150 (mean ± SD)
Cytokines	G₀	G₁	S + G₂/M
0 h culture time	28.3 ± 5.8	69.4 ± 6.2	1.9 ± 0.9
48 h culture time
SDF-1⁻
No cytokine	21.5 ± 5.1	72.2 ± 3.1	6.8 ± 1.4
SF	25.3 ± 5.1	52.4 ± 2.5	20.1 ± 3.4^‡
Tpo	20.8 ± 4.2	67.4 ± 2.8	13.1 ± 3.2^4-151
Flt-3 ligand	28.8 ± 4.8	63.8 ± 4.8	8.2 ± 3.5
SDF-1⁺
No cytokine	6.4 ± 1.8^‡	81.8 ± 4.1	11.2 ± 2.1
SF	10.5 ± 3.2^4-151	66.3 ± 2.9	22.3 ± 2.4^4-153
Tpo	8.4 ± 2.1^‡	63.8 ± 3.1	24.1 ± 3.9^4-153
Flt-3 ligand	9.1 ± 2.8	79.7 ± 3.5	12.4 ± 2.8

PB CD34⁺ cells purified immediately after density gradient separation (Inc⁻) were incubated (1 × 10⁵ cells/mL) in a serum-free StemαA medium in presence or absence of Tpo (10 ng/mL), SF (50 ng/mL), Flt-3 ligand (50 ng/mL), SDF-1 (0.5 ng/mL) used alone, or in combination. At various time points, cells were harvested, counted, and dually stained with PI and anti-Ki67-FITC mAb before flow cytometry analysis.

F4-150

Based on 3 independent experiments.

F4-151

P < .003 and

‡

P < .0001 versus no factor.

F4-153

P < .003 versus SDF-1 alone.

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