Table 1.

Allogeneic day 15/16 gestational fetal liver cells have a competitive engraftment advantage over adult bone marrow cells when given to fetal recipients

CD4+CD8+CD19+MAC-1+Total
2 mo      
 % Donor BM origin 3.9 ± 0.4 1.1 ± 0.1 3.5 ± 0.2 0.0 ± 0.0 8.6 ± 0.5 
 % Donor FL origin 35.8 ± 1.1 13.4 ± 3.2 25.0 ± 1.3 4.3 ± 0.3 78.5 ± 1.9 
4.5 mo      
 % Donor BM origin 2.8 ± 0.2 1.3 ± 0.1 3.6 ± 0.3 0.04 ± 0.04 7.8 ± 0.4 
 % Donor FL origin 28.2 ± 0.2 15.8 ± 0.5 23.4 ± 1.6 5.1 ± 0.3 72.4 ± 1.9 
12.5 mo      
 % Donor BM origin 1.7 ± 0.4 0.9 ± 0.2 8.3 ± 1.7 0.0 ± 0.0 10.9 ± 1.9 
 % Donor FL origin 17.4 ± 1.9 12.8 ± 1.3 35.1 ± 2.9 8.4 ± 0.9 73.7 ± 2.5 
CD4+CD8+CD19+MAC-1+Total
2 mo      
 % Donor BM origin 3.9 ± 0.4 1.1 ± 0.1 3.5 ± 0.2 0.0 ± 0.0 8.6 ± 0.5 
 % Donor FL origin 35.8 ± 1.1 13.4 ± 3.2 25.0 ± 1.3 4.3 ± 0.3 78.5 ± 1.9 
4.5 mo      
 % Donor BM origin 2.8 ± 0.2 1.3 ± 0.1 3.6 ± 0.3 0.04 ± 0.04 7.8 ± 0.4 
 % Donor FL origin 28.2 ± 0.2 15.8 ± 0.5 23.4 ± 1.6 5.1 ± 0.3 72.4 ± 1.9 
12.5 mo      
 % Donor BM origin 1.7 ± 0.4 0.9 ± 0.2 8.3 ± 1.7 0.0 ± 0.0 10.9 ± 1.9 
 % Donor FL origin 17.4 ± 1.9 12.8 ± 1.3 35.1 ± 2.9 8.4 ± 0.9 73.7 ± 2.5 

Balb/c severe combined immunodeficient fetuses at day 15/16 of gestation were injected intraperitoneally with 1 × 106 T-cell-depleted adult C57BL/6 CD45.2 bone marrow (BM) cells and 1 × 106 C57BL/6 CD45.1 fetal liver (FL) cells harvested at day 15/16 of gestation. Peripheral blood leukocytes were phenotyped at the indicated times after birth. The average percentage ± 1 SEM of cells resulting from each of the allogeneic stem cell sources is shown for each of the indicated cell lineages. n = 34 at 2 and 4.5 mo, n = 12 at 12.5 mo.

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